The effect of silver nanoparticles and silver ions on mammalian and plant cells in vitro
Language English Country Great Britain, England Media print-electronic
Document type Journal Article
PubMed
27456126
DOI
10.1016/j.fct.2016.07.015
PII: S0278-6915(16)30235-6
Knihovny.cz E-resources
- Keywords
- Genotoxicity, In vitro cytotoxicity, Mammalian cell, Silver ions, Silver nanoparticles, Solanum spp.,
- MeSH
- Apoptosis drug effects MeSH
- Comet Assay MeSH
- Metal Nanoparticles administration & dosage chemistry MeSH
- Cells, Cultured MeSH
- Humans MeSH
- Mice MeSH
- Oxidation-Reduction MeSH
- Lipid Peroxidation drug effects MeSH
- DNA Damage drug effects MeSH
- Cell Proliferation drug effects MeSH
- Spectrum Analysis, Raman MeSH
- Reactive Oxygen Species metabolism MeSH
- Solanum lycopersicum cytology drug effects MeSH
- Silver chemistry pharmacology MeSH
- In Vitro Techniques MeSH
- Blotting, Western MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Reactive Oxygen Species MeSH
- Silver MeSH
Silver nanoparticles (AgNPs) are the most frequently applied nanomaterials. In our experiments, we tested AgNPs (size 27 nm) manufactured by the Tollens process. Physico-chemical methods (TEM, DLS, AFM and spectrophotometry) were used for characterization and imaging of AgNPs. The effects of AgNPs and Ag(+) were studied in two experimental models (plant and mammalian cells). Human keratinocytes (SVK14) and mouse fibroblasts (NIH3T3) cell lines were selected to evaluate the cytotoxicity and genotoxicity effect on mammalian cells. Higher sensitivity to AgNPs and Ag(+) was observed in NIH3T3 than in SVK14 cells. AgNPs accumulated in the nucleus of NIH3T3 cells, caused DNA damage and increased the number of apoptotic and necrotic cells. Three genotypes of Solanum spp. (S. lycopersicum cv. Amateur, S. chmielewskii, S. habrochaites) were selected to test the toxicity of AgNPs and Ag(+) on the plant cells. The highest values of peroxidase activity and lipid peroxidation were recorded after the treatment of S. habrochaites genotype with AgNPs. Increased ROS levels were likely the reason for observed damaged membranes in S. habrochaites. We found that the cytotoxic and genotoxic effects of AgNPs depend not only on the characteristics of nanoparticles, but also on the type of cells that are treated with AgNPs.
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