Somatic embryogenesis (SE) is an important biotechnological technique used for the propagation of many pine species in vitro. However, in bog pine, one of the most endangered tree species in the Czech Republic, limitations were observed, which negatively influenced the development and further germination of somatic embryos. Although initiation frequency was very low-0.95 %, all obtained cell lines were subjected to maturation. The best responding cell line (BC1) was used and subjected to six different variants of the maturation media. The media on which the highest number of early-precotyledonary/cotyledonary somatic embryos was formed was supplemented with 121 μM abscisic acid (ABA) and with 6 % maltose. In the end of maturation experiments, different abnormalities in formation of somatic embryos were observed. For visualization and identification of abnormalities in meristem development during proliferation and maturation processes, the environmental scanning electron microscope was used. In comparison to the classical light microscope, the non-commercial environmental scanning electron microscope AQUASEM II has been found as a very useful tool for the quick recognition of apical meristem disruption and abnormal development. To our knowledge, this is the first report discussing somatic embryogenesis in bog pine. Based on this observation, the cultivation procedure could be enhanced and the method for SE of bog pine optimized.

Faculty of AgriSciences Mendel University in Brno Zemědělská 1 Brno 613 00 Czech Republic

Institute of Scientific Instruments Academy of Sciences of the Czech Republic Královopolská 147 Brno 61264 Czech Republic

Zobrazit více v PubMed

New Phytol. 2008;177(2):356-66 PubMed

Protoplasma. 2010 Oct;246(1-4):89-99 PubMed

Micron. 1995;26(2):141-4 PubMed

Micron. 2016 May;84:67-71 PubMed

Methods Mol Biol. 2009;547:35-52 PubMed

J Exp Bot. 2014 Mar;65(5):1343-60 PubMed

Plant Cell Rep. 1989 Apr;8(4):203-6 PubMed

Microsc Res Tech. 2015 Jan;78(1):13-21 PubMed

Microsc Microanal. 2013 Aug;19(4):914-8 PubMed

Tree Physiol. 2009 Jan;29(1):147-56 PubMed

Plant Cell Rep. 1985 Aug;4(4):177-9 PubMed

Tree Physiol. 2012 Feb;32(2):232-44 PubMed

Ontogenez. 2014 Mar-Apr;45(2):112-20 PubMed

Mathematical-Physics Analyses of the Nozzle Shaping at the Aperture Gas Outlet into Free Space under ESEM Pressure Conditions

Comparative Analysis of Supersonic Flow in Atmospheric and Low Pressure in the Region of Shock Waves Creation for Electron Microscopy

Silicon triggers sorghum root enzyme activities and inhibits the root cell colonization by Alternaria alternata

In-situ preparation of plant samples in ESEM for energy dispersive x-ray microanalysis and repetitive observation in SEM and ESEM