Two Clusters of Fluoroquinolone and Clindamycin-Resistant Clostridium difficile PCR Ribotype 001 Strain Recognized by Capillary Electrophoresis Ribotyping and Multilocus Variable Tandem Repeat Analysis
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články
PubMed
27860531
DOI
10.1089/mdr.2016.0159
Knihovny.cz E-zdroje
- Klíčová slova
- Clostridium difficile, MLVA, PCR ribotype 001, Thr82Ile, antimicrobial drug resistance, capillary electrophoresis ribotyping,
- MeSH
- antibakteriální látky farmakologie MeSH
- centra terciární péče MeSH
- ciprofloxacin farmakologie MeSH
- Clostridioides difficile klasifikace účinky léků genetika izolace a purifikace MeSH
- DNA bakterií genetika metabolismus MeSH
- DNA gyráza genetika metabolismus MeSH
- elektroforéza kapilární MeSH
- exprese genu MeSH
- fluorochinolony farmakologie MeSH
- klindamycin farmakologie MeSH
- klostridiové infekce farmakoterapie mikrobiologie MeSH
- lidé MeSH
- methyltransferasy genetika metabolismus MeSH
- metronidazol farmakologie MeSH
- mikrobiální testy citlivosti MeSH
- minisatelitní repetice MeSH
- mnohočetná bakteriální léková rezistence genetika MeSH
- moxifloxacin MeSH
- multilokusová sekvenční typizace MeSH
- retrospektivní studie MeSH
- ribotypizace MeSH
- senioři MeSH
- substituce aminokyselin MeSH
- vankomycin farmakologie MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Česká republika MeSH
- Názvy látek
- antibakteriální látky MeSH
- ciprofloxacin MeSH
- DNA bakterií MeSH
- DNA gyráza MeSH
- fluorochinolony MeSH
- klindamycin MeSH
- methyltransferasy MeSH
- metronidazol MeSH
- moxifloxacin MeSH
- rRNA (adenosine-O-2'-)methyltransferase MeSH Prohlížeč
- vankomycin MeSH
AIM: To perform a retrospective analysis of the high occurrence of Clostridium difficile infection in the surgical department of a Czech tertiary care hospital and to identify weaknesses in C. difficile infection (CDI) prevention and control policies. METHODS: Clinical and epidemiological data on eleven CDI cases were collected. C. difficile isolates were characterized by capillary electrophoresis ribotyping, multilocus variable tandem repeat analysis (MLVA), gyrA gene fragment sequencing, and erm(B) fragment PCR amplification. Antibiotic susceptibility to metronidazole, vancomycin, ciprofloxacin, moxifloxacin, and clindamycin was tested. FINDINGS: Eleven CDI cases were caused by C. difficile PCR ribotype 001 strains. These strains revealed two different MLVA profiles with 11 tandem repeat differences. All isolates were susceptible to metronidazole and vancomycin and resistant to ciprofloxacin (MIC ≥32 mg/L), moxifloxacin (MIC ≥32 mg/L), and clindamycin (MIC ≥256 mg/L). All isolates revealed amino acid substitution Thr82Ile, in the GyrA and were erm(B) negative. CONCLUSION: Two fluoroquinolone and clindamycin-resistant C. difficile PCR ribotype 001 strain clusters occurred at one of the surgical departments of a tertiary care hospital. Ineffective decontamination with suboptimal concentration and time of exposure of sporicidal disinfectants may have resulted in C. difficile transmission.
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