Tri- and tetravalent mannoclusters cross-link and aggregate BC2L-A lectin from Burkholderia cenocepacia
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články
PubMed
27871013
DOI
10.1016/j.carres.2016.11.008
PII: S0008-6215(16)30316-0
Knihovny.cz E-zdroje
- Klíčová slova
- Anti-adhesion therapy, Burkholderia cenocepacia, Click reaction, Glycoclusters, Mannose-binding lectin,
- MeSH
- aglutinační testy MeSH
- Burkholderia cenocepacia chemie MeSH
- kalorimetrie metody MeSH
- kvasinky účinky léků MeSH
- lektin vázající mannosu chemie metabolismus farmakologie MeSH
- ligandy MeSH
- mannosidy chemická syntéza chemie metabolismus MeSH
- povrchová plasmonová rezonance MeSH
- techniky syntetické chemie MeSH
- ultracentrifugace metody MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- lektin vázající mannosu MeSH
- ligandy MeSH
- mannosidy MeSH
The opportunistic Gram-negative bacterium Burkholderia cenocepacia causes lethal infections in cystic fibrosis patients. Multivalent mannoside derivatives were prepared as potential inhibitors of lectin BC2L-A, one of the virulence factors deployed by B. cenocepacia in the infection process. An (α1→2)-thio-linked mannobioside mimic bearing an azide functionalized aglycon was conjugated to different multivalent scaffolds such as propargylated calix[4]arenes, methyl gallate and pentaerythritol by azide-alkyne 1,3-dipolar cycloaddition. The interaction between the glycoclusters and the mannose binding BC2L-A lectin from B. cenocepacia was examined by isothermal microcalorimetry, surface plasmon resonance, inhibition of yeast agglutination and analytical ultracentrifugation.
Central European Institute of Technology Masaryk University Kamenice 5 625 00 Brno Czechia
Department of Organic Chemistry University of Debrecen POB 20 H 410 Debrecen Hungary
Department of Pharmaceutical Chemistry University of Debrecen POB 78 H 4010 Debrecen Hungary
ICBMS Equipe CSAp Université Lyon 1 69622 Villeurbanne France
Citace poskytuje Crossref.org