Effect of 24-epibrassinolide on Brassica napus alternative respiratory pathway, guard cells movements and phospholipid signaling under salt stress
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
27913097
DOI
10.1016/j.steroids.2016.11.006
PII: S0039-128X(16)30178-7
Knihovny.cz E-resources
- Keywords
- Alternative oxidase, Brassica napus, Brassinosteroids, Diacylglycerol, Salinity, Stomata cells,
- MeSH
- Brassica napus drug effects enzymology metabolism MeSH
- Brassinosteroids pharmacology MeSH
- Diglycerides metabolism MeSH
- Plant Roots drug effects enzymology metabolism MeSH
- Phosphatidic Acids metabolism MeSH
- Plant Leaves drug effects enzymology metabolism MeSH
- Mitochondrial Proteins metabolism MeSH
- Oxidoreductases metabolism MeSH
- Plant Stomata drug effects enzymology metabolism MeSH
- Plant Proteins metabolism MeSH
- Steroids, Heterocyclic pharmacology MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- alternative oxidase MeSH Browser
- brassinolide MeSH Browser
- Brassinosteroids MeSH
- Diglycerides MeSH
- Phosphatidic Acids MeSH
- Mitochondrial Proteins MeSH
- Oxidoreductases MeSH
- Plant Proteins MeSH
- Steroids, Heterocyclic MeSH
Using Brassica napus roots we observed statistically significant increase in alternative respiratory pathway in response to exogenous 24-epibrassinolide (EBL) under optimal conditions and salinity. Also we observed activation of phospholipid signaling under the same conditions in response to EBL by measuring levels of lipid second messengers - diacylglycerol (DAG) and phosphatidic acid (PA). We found that brassinosteroids cause closure of stomata in isolated leaf disks while inhibitors of alternative oxidase cancelled these effects. This study demonstrates that BRs activate total respiration rate, alternative respiratory pathway, production of PA and DAG, stimulate stomata closure and growth under optimal conditions and salinity. Also, specific inhibitor of brassinosteroids biosynthesis decreased alternative respiratory pathway and production of lipid messengers in rape plants.
References provided by Crossref.org
Influence of Exogenous 24-Epicasterone on the Hormonal Status of Soybean Plants
Phosphatidic Acid in Plant Hormonal Signaling: From Target Proteins to Membrane Conformations
