Using Brassica napus roots we observed statistically significant increase in alternative respiratory pathway in response to exogenous 24-epibrassinolide (EBL) under optimal conditions and salinity. Also we observed activation of phospholipid signaling under the same conditions in response to EBL by measuring levels of lipid second messengers - diacylglycerol (DAG) and phosphatidic acid (PA). We found that brassinosteroids cause closure of stomata in isolated leaf disks while inhibitors of alternative oxidase cancelled these effects. This study demonstrates that BRs activate total respiration rate, alternative respiratory pathway, production of PA and DAG, stimulate stomata closure and growth under optimal conditions and salinity. Also, specific inhibitor of brassinosteroids biosynthesis decreased alternative respiratory pathway and production of lipid messengers in rape plants.

Department of Biology Faculty of Sciences Jan Evangelista Purkyne University Usti nad Labem Czech Republic

Department of the Molecular Mechanisms of Cell Metabolism Regulation Institute of Bioorganic Chemistry and Petrochemistry The National Academy of Sciences of Ukraine 02660 Murmanska str 1 Kyiv Ukraine

Institute of Food Biotechnology and Genomics National Academy of Sciences of Ukraine Osypovskogo 2a Kyiv 04123 Ukraine

Laboratory of Steroid Chemistry Institute of Bioorganic Chemistry The National Academy of Sciences of Belarus 220141 Kuprevich str 5 Minsk Belarus

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