Strains of Bifidobacterium animalis subsp. lactis are well-known health-promoting probiotics used commercially. B. animalis subsp. lactis has been isolated from different sources, and little is known about animal isolates of this taxon. The aim of this study was to examine the genotypic and phenotypic diversity between B. animalis subsp. lactis strains different animal hosts including Cameroon sheep, Barbary sheep, okapi, mouflon, German shepard and to compare to BB12, food isolates and the collection strain DSM 10140. Ten strains of B. animalis subsp. lactis from different sources were characterised by phenotyping, fingerprinting, and multilocus sequence typing (MLST). Regardless of origin, MLST and phylogenetic analyses revealed a close relationship between strains of B. animalis subsp. lactis with commercial and animal origin with the exception of isolates from ovine cheese, mouflon and German Shepard dog. Moreover, isolates from dog and mouflon showed significant differences in fermentation profiles and peptide mass fingerprints (MALDI-TOF). Results indicated phenotypic and genotypic diversity among strains of B. animalis subsp. lactis.

Department of Biochemistry and Microbiology Institute of Chemical Technology Prague Technická 5 166 28 Prague Czechia

Department of Microbiology Nutrition and Dietetics Czech University of Life Sciences Prague Kamýcka 129 Prague 6 165 21 Czechia

Department of Microbiology Nutrition and Dietetics Czech University of Life Sciences Prague Kamýcka 129 Prague 6 165 21 Czechia; Institute of Animal Physiology and Genetics Academy of Sciences of the Czech Republic Vídeňská 1083 Prague 4 Krč 14 200 Czechia

Department of Soil Science and Soil Protection Czech University of Life Sciences Prague Kamýcká 129 165 21 Prague 6 Czechia

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