Beneficial effect of maternal milk is acknowledged, but there is still question whether maternal milk from allergic mother is as good as from healthy one. In our study, we have assayed the effect of cells from colostrum of healthy and allergic mothers on gene expression of cytokines in cord blood cells of newborns of healthy and allergic mothers. Cytokines typical for Th1 (IL-2, IFN-gamma), Th2 (IL-4, IL-13), Tregs (IL-10, TGF-beta), and IL-8 were followed. We were not able to detect significant influence of colostral cells on gene expression of cytokines in cord blood after 2-day coculture using Transwell system. There was no difference in gene expression of cytokines in nonstimulated cord blood cells of newborns of healthy and allergic mothers, but generally increased gene expression of cytokines except IL-10 and TGF-beta after polyclonal stimulation was detected in cord blood cells of children of allergic mothers. There was no difference in IL-10 expression in stimulated cord blood cells of children of healthy and allergic mothers. Gene expression of TGF-beta was even decreased in stimulated cord blood cells of children of allergic mothers in comparison to healthy ones. We have not observed difference in the capacity of colostral cells of healthy and allergic mothers to influence gene expression of cytokines in cord blood cells, but we have described difference in the reactivity of cord blood cells between children of allergic and healthy mothers.

Institute for the Care of Mother and Child Prague Czech Republic

Institute of Immunology and Microbiology 1st Faculty of Medicine and General UniversityHospital Prague Charles University Studničkova 7 128 00 Prague Czech Republic

Zobrazit více v PubMed

Vet Immunol Immunopathol. 2008 Jun 15;123(3-4):305-13 PubMed

Clin Exp Immunol. 2012 Oct;170(1):10-7 PubMed

Folia Microbiol (Praha). 2012 May;57(3):215-9 PubMed

Pediatr Allergy Immunol. 2013 Mar;24(2):178-86 PubMed

Clin Exp Allergy. 1998 Nov;28(11):1340-4 PubMed

J Reprod Immunol. 1984 Aug;6(5):329-37 PubMed

Pediatr Allergy Immunol. 2012 May;23(3):210-23 PubMed

Allergy. 2008 Nov;63(11):1438-47 PubMed

Folia Biol (Praha). 2014;60(2):74-82 PubMed

Arch Dis Child. 1989 Jul;64(7 Spec No):930-3 PubMed

Vet Immunol Immunopathol. 2002 Feb;85(1-2):33-40 PubMed

Allergy. 2003 Nov;58(11):1187-94 PubMed

Lancet. 1977 Sep 10;2(8037):529-32 PubMed

Folia Microbiol (Praha). 2010 Sep;55(5):515-9 PubMed

Pediatr Allergy Immunol. 2007 Sep;18(6):486-94 PubMed

Lancet. 1972 Mar 25;1(7752):688 PubMed

Pediatr Allergy Immunol. 2014 Nov;25(7):674-84 PubMed

Asian Pac J Allergy Immunol. 2014 Mar;32(1):84-92 PubMed

J Allergy Clin Immunol. 2017 Aug;140(2):534-542 PubMed

J Allergy Clin Immunol. 2003 Dec;112(6):1072-7 PubMed

Effect of early postnatal supplementation of newborns with probiotic strain E. coli O83:K24:H31 on allergy incidence, dendritic cells, and microbiota

Different immune response of dendritic cells of newborns of allergic and healthy mothers to bacterial stimuli