Inducible protein stabilization system in Leishmania mexicana
Language English Country Netherlands Media print-electronic
Document type Journal Article
PubMed
28373094
DOI
10.1016/j.molbiopara.2017.03.008
PII: S0166-6851(17)30046-4
Knihovny.cz E-resources
- Keywords
- Leishmania mexicana, Protein stabilization, Trimethoprim, ecDHFR,
- MeSH
- Transcriptional Activation * MeSH
- Tetrahydrofolate Dehydrogenase genetics metabolism MeSH
- Escherichia coli enzymology genetics MeSH
- Leishmania mexicana genetics metabolism MeSH
- Molecular Biology methods MeSH
- Parasitology methods MeSH
- Gene Expression Regulation * MeSH
- Recombinant Proteins genetics metabolism MeSH
- Trimethoprim metabolism MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Tetrahydrofolate Dehydrogenase MeSH
- Recombinant Proteins MeSH
- Trimethoprim MeSH
Targeted regulation of protein levels is an important tool to investigate the role of proteins essential for cell function and development. In recent years, methods based on the Escherichia coli dihydrofolate reductase destabilization domain (ecDHFR DD) have been established and used in various cell types. ecDHFR DD destabilizes the fused protein of interest and causes its degradation by proteasomes, unless it is stabilized by a specific ligand, trimethoprim. In this work we developed an inducible protein stabilization system in Leishmania mexicana based on ecDHFR DD.
Department of Pathology Albert Einstein College of Medicine Bronx NY 10461 USA
Life Science Research Centre Faculty of Science University of Ostrava 710 00 Ostrava Czech Republic
References provided by Crossref.org
Catalase impairs Leishmania mexicana development and virulence
