Inducible protein stabilization system in Leishmania mexicana
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články
PubMed
28373094
DOI
10.1016/j.molbiopara.2017.03.008
PII: S0166-6851(17)30046-4
Knihovny.cz E-zdroje
- Klíčová slova
- Leishmania mexicana, Protein stabilization, Trimethoprim, ecDHFR,
- MeSH
- aktivace transkripce * MeSH
- dihydrofolátreduktasa genetika metabolismus MeSH
- Escherichia coli enzymologie genetika MeSH
- Leishmania mexicana genetika metabolismus MeSH
- molekulární biologie metody MeSH
- parazitologie metody MeSH
- regulace genové exprese * MeSH
- rekombinantní proteiny genetika metabolismus MeSH
- trimethoprim metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- dihydrofolátreduktasa MeSH
- rekombinantní proteiny MeSH
- trimethoprim MeSH
Targeted regulation of protein levels is an important tool to investigate the role of proteins essential for cell function and development. In recent years, methods based on the Escherichia coli dihydrofolate reductase destabilization domain (ecDHFR DD) have been established and used in various cell types. ecDHFR DD destabilizes the fused protein of interest and causes its degradation by proteasomes, unless it is stabilized by a specific ligand, trimethoprim. In this work we developed an inducible protein stabilization system in Leishmania mexicana based on ecDHFR DD.
Department of Pathology Albert Einstein College of Medicine Bronx NY 10461 USA
Life Science Research Centre Faculty of Science University of Ostrava 710 00 Ostrava Czech Republic
Citace poskytuje Crossref.org
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