Remodeling of tick cytoskeleton in response to infection with Anaplasma phagocytophilum
Jazyk angličtina Země Singapur Médium electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
28410148
DOI
10.2741/4574
PII: 4574
Knihovny.cz E-zdroje
- MeSH
- aktiny genetika metabolismus MeSH
- Anaplasma phagocytophilum fyziologie MeSH
- buněčné linie MeSH
- cytoskelet genetika metabolismus mikrobiologie MeSH
- fylogeneze MeSH
- interakce hostitele a patogenu MeSH
- klíště genetika metabolismus mikrobiologie MeSH
- konfokální mikroskopie MeSH
- polymerázová řetězová reakce s reverzní transkripcí MeSH
- proteiny členovců genetika metabolismus MeSH
- proteomika metody MeSH
- septiny klasifikace genetika metabolismus MeSH
- stanovení celkové genové exprese metody MeSH
- tubulin genetika metabolismus MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- aktiny MeSH
- proteiny členovců MeSH
- septiny MeSH
- tubulin MeSH
The obligate intracellular pathogen Anaplasma phagocytophilum infects vertebrate and tick hosts. In this study, a genome-wide search for cytoskeleton components was performed in the tick vector, Ixodes scapularis. The available transcriptomics and proteomics data was then used to characterize the mRNA and protein levels of I. scapularis cytoskeleton components in response to A. phagocytophilum infection. The results showed that cytoskeleton components described in other model organisms were present in the I. scapularis genome. One type of intermediate filaments (lamin), a family of septins that was recently implicated in the cellular response to intracellular pathogens, and several members of motor proteins (kinesins and dyneins) that could be implicated in the cytoplasmic movements of A. phagocytophilum were found. The results showed that levels of tubulin, actin, septin, actin-related proteins and motor proteins were affected by A. phagocytophilum, probably to facilitate infection in I. scapularis. Functional studies demonstrated a role for selected cytoskeleton components in pathogen infection. These results provided a more comprehensive view of the cytoskeletal components involved in the response to A. phagocytophilum infection in ticks.
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