Remodeling of tick cytoskeleton in response to infection with Anaplasma phagocytophilum
Language English Country Singapore Media electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
28410148
DOI
10.2741/4574
PII: 4574
Knihovny.cz E-resources
- MeSH
- Actins genetics metabolism MeSH
- Anaplasma phagocytophilum physiology MeSH
- Cell Line MeSH
- Cytoskeleton genetics metabolism microbiology MeSH
- Phylogeny MeSH
- Host-Pathogen Interactions MeSH
- Ixodes genetics metabolism microbiology MeSH
- Microscopy, Confocal MeSH
- Reverse Transcriptase Polymerase Chain Reaction MeSH
- Arthropod Proteins genetics metabolism MeSH
- Proteomics methods MeSH
- Septins classification genetics metabolism MeSH
- Gene Expression Profiling methods MeSH
- Tubulin genetics metabolism MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Actins MeSH
- Arthropod Proteins MeSH
- Septins MeSH
- Tubulin MeSH
The obligate intracellular pathogen Anaplasma phagocytophilum infects vertebrate and tick hosts. In this study, a genome-wide search for cytoskeleton components was performed in the tick vector, Ixodes scapularis. The available transcriptomics and proteomics data was then used to characterize the mRNA and protein levels of I. scapularis cytoskeleton components in response to A. phagocytophilum infection. The results showed that cytoskeleton components described in other model organisms were present in the I. scapularis genome. One type of intermediate filaments (lamin), a family of septins that was recently implicated in the cellular response to intracellular pathogens, and several members of motor proteins (kinesins and dyneins) that could be implicated in the cytoplasmic movements of A. phagocytophilum were found. The results showed that levels of tubulin, actin, septin, actin-related proteins and motor proteins were affected by A. phagocytophilum, probably to facilitate infection in I. scapularis. Functional studies demonstrated a role for selected cytoskeleton components in pathogen infection. These results provided a more comprehensive view of the cytoskeletal components involved in the response to A. phagocytophilum infection in ticks.
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