Barley (Hordeum vulgare L.) is a cereal grass mainly used as animal fodder and raw material for the malting industry. The map-based reference genome sequence of barley cv. 'Morex' was constructed by the International Barley Genome Sequencing Consortium (IBSC) using hierarchical shotgun sequencing. Here, we report the experimental and computational procedures to (i) sequence and assemble more than 80,000 bacterial artificial chromosome (BAC) clones along the minimum tiling path of a genome-wide physical map, (ii) find and validate overlaps between adjacent BACs, (iii) construct 4,265 non-redundant sequence scaffolds representing clusters of overlapping BACs, and (iv) order and orient these BAC clusters along the seven barley chromosomes using positional information provided by dense genetic maps, an optical map and chromosome conformation capture sequencing (Hi-C). Integrative access to these sequence and mapping resources is provided by the barley genome explorer (BARLEX).

Australian Export Grains Innovation Centre South Perth Western Australia 6151 Australia

BGI Shenzhen Shenzhen 518083 China

BioNano Genomics Inc San Diego California 92121 USA

Carlsberg Research Laboratory 1799 Copenhagen Denmark

Centre for Comparative Genomics Murdoch University Murdoch Western Australia 6150 Australia

College of Agriculture and Biotechnology Zhejiang University Hangzhou 310058 China

Department of Agricultural and Environmental Sciences University of Udine 33100 Udine Italy

Department of Agriculture and Food Government of Western Australia South Perth Western Australia 6150 Australia

Department of Agronomy and Plant Genetics University of Minnesota St Paul Minnesota 55108 USA

Department of Biology Lund University 22362 Lund Sweden

Department of Botany and Plant Sciences University of California Riverside Riverside California 92521 USA

Department of Computer Science and Engineering University of California Riverside Riverside California 92521 USA

Department of Plant and Microbial Biology University of Minnesota St Paul Minnesota 55108 USA

Earlham Institute Norwich NR4 7UH UK

European Molecular Biology Laboratory The European Bioinformatics Institute Hinxton CB10 1SD UK

German Centre for Integrative Biodiversity Research Halle Jena Leipzig 04103 Leipzig Germany

Green Technology Natural Resources Institute Viikki Plant Science Centre and Institute of Biotechnology University of Helsinki 00014 Helsinki Finland

Hubei Collaborative Innovation Centre for Grain Industry Yangtze University Jingzhou Hubei 434025 China

Institute of Experimental Botany Centre of the Region Haná for Biotechnological and Agricultural Research 78371 Olomouc Czech Republic

Kansas State University Wheat Genetics Resource Center Department of Plant Pathology and Department of Agronomy Manhattan Kansas 66506 USA

Leibniz Institute of Plant Genetics and Crop Plant Research Gatersleben 06466 Seeland Germany

Leibniz Institute on Aging Fritz Lipmann Institute 07745 Jena Germany

National Institute of Agricultural Botany Cambridge CB3 0LE UK

School of Agriculture University of Adelaide Urrbrae South Australia 5064 Australia

School of Environmental Sciences University of East Anglia Norwich NR4 7UH UK

School of Life Sciences University of Dundee Dundee DD2 5DA UK

School of Plant Biology University of Western Australia Crawley 6009 Australia

School of Veterinary and Life Sciences Murdoch University Murdoch Western Australia 6150 Australia

The James Hutton Institute Dundee DD2 5DA UK

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doi: 10.1111/tpj.12959 PubMed

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