For many years initiation and termination of mRNA translation have been studied separately. However, a direct link between these 2 isolated stages has been suggested by the fact that some initiation factors also control termination and can even promote ribosome recycling; i.e. the last stage where post-terminating 80S ribosomes are split to start a new round of initiation. Notably, it is now firmly established that, among other factors, ribosomal recycling critically requires the NTPase ABCE1. However, several earlier reports have proposed that ABCE1 also somehow participates in the initiation complex assembly. Based on an extended analysis of our recently published late-stage 48S initiation complex from rabbit, here we provide new mechanistic insights into this putative role of ABCE1 in initiation. This point of view represents the first structural evidence in which the regulatory role of the recycling factor ABCE1 in initiation is discussed and establishes a corner stone for elucidating the interplay between ABCE1 and several initiation factors during the transit from ribosomal recycling to formation of the elongation competent 80S initiation complex.

b Laboratory of Regulation of Gene Expression Institute of Microbiology ASCR Prague Czech Republic

CNRS Architecture et Réactivité de l'ARN UPR9002 Université de Strasbourg Strasbourg France

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Ribosomal A-site interactions with near-cognate tRNAs drive stop codon readthrough

uS3/Rps3 controls fidelity of translation termination and programmed stop codon readthrough in co-operation with eIF3

Does eIF3 promote reinitiation after translation of short upstream ORFs also in mammalian cells?

Embraced by eIF3: structural and functional insights into the roles of eIF3 across the translation cycle