In the present study, we used Illumina sequencing technology (HiSeq 2000) to sequence the transcriptome of barley (Hordeum vulgare L., cv. Morex) under three different heavy metal stress conditions: copper, zinc and cadmium. For each of those metals, the concentration causing a 50% inhibitory effect for root growth (EC50) was determined. We sequenced the total RNA of both roots and shoots from barley with and without heavy metal treatments in three replicates. Raw reads of the transcriptome project have been deposited in NCBI's BioProject accession number PRJNA382490. The obtained transcriptomic data will be useful for further studies focusing on heavy metal tolerance and comparative transcriptome analysis in barley.

Department of Plant Developmental Genetics Institute of Biophysics of the CAS v v i Kralovopolska 135 CZ 61200 Brno Czech Republic

Institute of Experimental Botany AS CR Centre of the Region Hana for Biotechnological and Agricultural Research Slechtitelu 31 CZ 78371 Olomouc Holice Czech Republic

Zobrazit více v PubMed

Hänsch R., Mendel R.R. Physiological functions of mineral micronutrients (Cu, Zn, Mn, Fe, Ni, Mo, B, Cl) Curr. Opin. Plant Biol. 2009;12:259–266. PubMed

Broadley M.R., White P.J., Hammond J.P., Zelko I., Lux A. Zinc in plants. New Phytol. 2007;173:677–702. PubMed

Dietz K.-J., Baier M., Krämer U. Heavy Metal Stress in Plants. Springer; Berlin Heidelberg: 1999. Free radicals and reactive oxygen species as mediators of heavy metal toxicity in plants; pp. 73–97.

Van Assche F., Clijsters H. Effects of metals on enzyme activity in plants. Plant Cell Environ. 1990;13:195–206.

John D.A., Leventhal J.S. Chapter 2 — bioavailability of metals. In: du Bray Edward A., editor. Preliminary Compilation of Descriptive Geoenvironmental Mineral Deposit Models. U.S. Geological Survey Open-File Book; Denver: 1996. pp. 10–18.

Cegan R., Hudzieczek V., Hobza R. De novo transcriptome assembly of heavy metal tolerant Silene dioica. Genomics Data. 2017;11:118–119. PubMed PMC

Proteome Analysis of Condensed Barley Mitotic Chromosomes

Transcriptome Response to Cadmium Exposure in Barley (Hordeum vulgare L.)