Over the last five decades, many methods to analyze thiamine (vitamin B1) and its phosphorylated forms in urine, whole blood, serum, plasma and erythrocytes have been proposed. Some of the methods are presently used in routine practice, but analytical problems regarding reproducibility, standardization, lack of automation, time consuming procedures for pretreatment and analysis are often discussed. With modern approaches to bioanalysis in clinical research of vitamins, whole processes can be automated, making analysis less time consuming, with reduced consumption of solvents and samples. This review critically discusses various analytical techniques, their advantages and disadvantages that are used for determination of thiamine and its derivatives in clinical practice, with emphasis on accurate, reliable and fast analytical procedures.

3rd Internal Gerontometabolic Clinic University Hospital Sokolská 581 Hradec Králové 500 05 Czech Republic

Department of Analytical Chemistry Charles University Faculty of Pharmacy in Hradec Králové Akademika Heyrovského 1203 Hradec Králové 500 05 Czech Republic

Department of Analytical Chemistry Charles University Faculty of Pharmacy in Hradec Králové Akademika Heyrovského 1203 Hradec Králové 500 05 Czech Republic; 3rd Internal Gerontometabolic Clinic University Hospital Sokolská 581 Hradec Králové 500 05 Czech Republic

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