Recent trends in determination of thiamine and its derivatives in clinical practice

. 2017 Aug 11 ; 1510 () : 1-12. [epub] 20170617

Jazyk angličtina Země Nizozemsko Médium print-electronic

Typ dokumentu časopisecké články, přehledy

Perzistentní odkaz   https://www.medvik.cz/link/pmid28652005
Odkazy

PubMed 28652005
DOI 10.1016/j.chroma.2017.06.048
PII: S0021-9673(17)30913-5
Knihovny.cz E-zdroje

Over the last five decades, many methods to analyze thiamine (vitamin B1) and its phosphorylated forms in urine, whole blood, serum, plasma and erythrocytes have been proposed. Some of the methods are presently used in routine practice, but analytical problems regarding reproducibility, standardization, lack of automation, time consuming procedures for pretreatment and analysis are often discussed. With modern approaches to bioanalysis in clinical research of vitamins, whole processes can be automated, making analysis less time consuming, with reduced consumption of solvents and samples. This review critically discusses various analytical techniques, their advantages and disadvantages that are used for determination of thiamine and its derivatives in clinical practice, with emphasis on accurate, reliable and fast analytical procedures.

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