Bioanalysis
Dotaz
Zobrazit nápovědu
sv. : il. ; 28 cm
- MeSH
- chemické techniky analytické * metody MeSH
- farmaceutická technologie MeSH
- léčivé přípravky analýza MeSH
- Publikační typ
- periodika MeSH
- Konspekt
- Farmacie. Farmakologie
- NLK Obory
- farmacie a farmakologie
1 elektronický optický disk
- MeSH
- klinické laboratorní techniky MeSH
- laboratoře MeSH
- řízení kvality MeSH
- Publikační typ
- kongresy MeSH
- Konspekt
- Lékařské vědy. Lékařství
- NLK Obory
- veřejné zdravotnictví
... 194 -- References 196 -- 10 MICROBIAL SENSORS 199 -- 10.1 Applications of Microbial Sensors in Bioanalysis ... ... for Electrochemical Sensor Arrays 208 -- 11.2 Applications of Electrochemical Sensor Arrays in Bioanalysis ... ... Electrodes in Flow Systems 248 -- 13.5 Applications of Electrochemical Sensor/FIA Systems in -- Bioanalysis ... ... 252 -- 13.5.2 Organic Ions 252 -- 13.6 Applications of Electrochemical Sensor/SIA Systems in -- Bioanalysis ... ... When Electrochemical Sensors -- Are Used in Bioanalysis 271 -- 15.5 /? ...
288 s.
Advanced metal deposition and microfabrication techniques enable preparation of metal surfaces with high precision and excellent control over their size and shape with subnanometer resolution. Thin metal films of different types and functions can be found in many analytical instruments. Surfaces with high optical quality serve as mirrors, beam splitters, antireflective coatings etc. Smooth metal coating is crucial in electron microscopy. Unique properties of the thin metal films are widely used in optical systems, as tools for sample manipulation but also for chemical sensing and detection. While some of the applications are widespread and belong to the basic curriculum in analytical chemistry, the newer or less common uses of thin metal films are well known only to the experts in the field. The purpose of this critical review is to highlight the role of thin metal films in bioanalysis and summarize some of their main applications in current bioanalytical instrumentation.
- MeSH
- biosenzitivní techniky přístrojové vybavení metody MeSH
- elektrochemické techniky přístrojové vybavení metody MeSH
- kovy chemie MeSH
- lidé MeSH
- mikrofluidika přístrojové vybavení metody MeSH
- nukleové kyseliny analýza MeSH
- proteiny analýza MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- přehledy MeSH
BACKGROUND: Matrix effects are considered to be a main obstacle of quantitative bioanalytical LC-MS/MS methods. Therefore it is often required to minimize them in order to increase method reliability. HILIC has been referenced as one of possible approaches. However, there is a lack of experimental evidence in scientific literature so far. METHODOLOGY: Matrix effects were evaluated using spiked serum samples after SPE and protein precipitation prior to UHPLC-ESI-MS/MS. Chromatography was performed in both HILIC and reversed-phase mode. The influence of the matrix effects on the signal response was assessed using a set of 34 compounds of pharmaceutical interest and post-extraction addition approach. RESULTS: The advantages and drawbacks of the HILIC and reversed-phase chromatographic modes were compared and discussed in detail. CONCLUSION: HILIC demonstrated the potential to reduce the occurrence of matrix effects when a more thorough sample pretreatment procedure such as SPE was applied.
- MeSH
- chromatografie s reverzní fází * MeSH
- extrakce na pevné fázi MeSH
- hmotnostní spektrometrie s elektrosprejovou ionizací MeSH
- hydrofobní a hydrofilní interakce MeSH
- krevní proteiny chemie MeSH
- léčivé přípravky krev MeSH
- rozpouštědla chemie MeSH
- tandemová hmotnostní spektrometrie * MeSH
- vysokoúčinná kapalinová chromatografie * MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
In 1957, protein rich in cysteine able to bind cadmium was isolated from horse kidney and named as metallothionein according to its structural properties. Further, this protein and metallothionein-like proteins have been found in tissues of other animal species, yeasts, fungi and plants. MT is as a potential cancer marker in the focus of interest, and its properties, functions, and behavior under various conditions are intensively studied. Our protocol describes separation of two major mammalian isoforms of MT (MT-1 and MT-2) using capillary electrophoresis (CE) coupled with UV detector. This protocol enables separation of MT isoforms and studying of their basic behavior as well as their quantification with detection limit in units of ng per μL. Sodium borate buffer (20 mM, pH 9.5) was optimized as a background electrolyte, and the separation was carried out in fused silica capillary with internal diameter of 75 μm and electric field intensity of 350 V/cm. Optimal detection wavelength was 254 nm.
