Species-specific real-time RT-PCR analysis of expression of stromal cell genes in a tumor xenotransplantation model in mice
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
28711492
DOI
10.1016/j.bbrc.2017.07.061
PII: S0006-291X(17)31403-1
Knihovny.cz E-resources
- Keywords
- CXCR4, Dipeptidyl peptidase, Fibroblast activation protein, Glioma, Species-specific expression, Tumor stroma,
- MeSH
- Stromal Cells metabolism MeSH
- Dipeptidyl-Peptidases and Tripeptidyl-Peptidases metabolism MeSH
- Species Specificity MeSH
- Glioma metabolism MeSH
- Real-Time Polymerase Chain Reaction MeSH
- Humans MeSH
- Brain metabolism MeSH
- Mice MeSH
- Cell Line, Tumor MeSH
- Brain Neoplasms metabolism MeSH
- Proteome metabolism MeSH
- Receptors, CXCR4 metabolism MeSH
- Gene Expression Regulation, Neoplastic * MeSH
- Gene Expression Profiling MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Dipeptidyl-Peptidases and Tripeptidyl-Peptidases MeSH
- Proteome MeSH
- Receptors, CXCR4 MeSH
Human tumor xenografts in mice together with the species-specific analysis of expressed genes allow to study the molecular processes driving tumor growth and progression in vivo and help to develop and evaluate anticancer therapies. In the present work, we designed and validated species-specific real-time RT-PCR assays for discrimination and quantitation of expression of human and mouse transcripts in cancer and stromal cells including dipeptidyl peptidase (DPP) 4, DPP8, DPP9, fibroblast activation protein (FAP) and CXC chemokine receptor 4 in mixed human-mouse biological samples. Using single species RNA samples and mixed human-mouse RNA samples, we formulated and characterized two-step real-time RT-PCR assays to quantitate expression of the indicated transcripts and described analytical performance of the assays. We also demonstrated the applicability of these assays for species-specific quantitation of transcriptional expression of mouse stromal cell genes including Dpp4, Dpp8, Dpp9, Fap and Cxcr4 in mixed human-mouse RNA samples from human glioma cell-derived tumor xenografts growing in mouse brain.
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