Effects of selected Indonesian plant extracts on E. cuniculi infection in vivo
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články
PubMed
28779899
DOI
10.1016/j.exppara.2017.07.014
PII: S0014-4894(17)30008-5
Knihovny.cz E-zdroje
- Klíčová slova
- Antifungal, Encephalitozoon cuniculi, In vivo, Molecular detection, Plant extract,
- MeSH
- albendazol farmakologie terapeutické užití MeSH
- antifungální látky farmakologie terapeutické užití MeSH
- Cercopithecus aethiops MeSH
- dimethylsulfoxid farmakologie terapeutické užití MeSH
- Diospyros chemie MeSH
- Dipterocarpaceae chemie MeSH
- DNA fungální izolace a purifikace MeSH
- Encephalitozoon cuniculi účinky léků MeSH
- encephalitozoonóza farmakoterapie MeSH
- Fabaceae chemie MeSH
- feces parazitologie MeSH
- gastrointestinální trakt mikrobiologie MeSH
- imunokompetence MeSH
- listy rostlin chemie MeSH
- myši inbrední BALB C MeSH
- myši MeSH
- Piper betle chemie MeSH
- rostlinné extrakty farmakologie terapeutické užití MeSH
- spory hub účinky léků MeSH
- Vero buňky MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Indonésie MeSH
- Názvy látek
- albendazol MeSH
- antifungální látky MeSH
- dimethylsulfoxid MeSH
- DNA fungální MeSH
- rostlinné extrakty MeSH
The present study was conducted to evaluate the methanolic extracts from several plant leaves widely used in traditional medicine to cure digestive tract disorders and in the self-medication of wild animals such as non-human primates, namely Archidendron fagifolium, Diospyros sumatrana, Shorea sumatrana, and Piper betle leaves, with regard to their antimicrosporidial activity against Encephalitozoon cuniculi in immunocompetent BALB/c mice determined using molecular detection of microsporidial DNA (qPCR) in various tissues and body fluids of infected, treated mice. Of the plant extracts tested, Diospyros sumatrana provided the most promising results, reducing spore shedding by 88% compared to untreated controls. Moreover, total burden per 1 g of tissue in the D. sumatrana extract-treated group reached 87% reduction compared to untreated controls, which was comparable to the effect of the standard drug, Albendazole. This data represents the baseline necessary for further research focused on determining the structure, activity and modes of action of the active compounds, mainly of D. sumatrana, enabling subsequent development of antimicrosporidial remedies.
Faculty of Economics University of South Bohemia in České Budějovice České Budějovice Czech Republic
Institute of Parasitology Biology Centre Czech Academy of Sciences České Budějovice Czech Republic
Citace poskytuje Crossref.org
Effect of selected Indonesian plants on Giardia intestinalis in an experimental in vitro model
Encephalitozoon cuniculi Genotype II Concentrates in Inflammation Foci
