Rapid Gene Isolation Using MutChromSeq
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
Grant support
Biotechnology and Biological Sciences Research Council - United Kingdom
- Keywords
- Chromosome isolation, FISHIS, Flow cytometry and chromosome sorting, Gene cloning, Mutational genomics,
- MeSH
- Chromosomes, Plant genetics MeSH
- DNA, Plant genetics MeSH
- Genome, Plant MeSH
- Genomics methods MeSH
- In Situ Hybridization, Fluorescence methods MeSH
- Mutation * MeSH
- Polyploidy MeSH
- Flow Cytometry methods MeSH
- Gene Expression Regulation, Plant MeSH
- Plants genetics MeSH
- Sequence Analysis, DNA methods MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA, Plant MeSH
MutChromSeq is an approach for isolation of genes and DNA sequences controlling gene expression in plants with complex and polyploid genomes. It involves a lossless complexity reduction by flow cytometric chromosome sorting and shotgun sequencing DNA from isolated chromosomes. Comparison of sequences from wild-type parental chromosome with chromosomes from multiple independently derived mutants identifies causative mutations in a single candidate gene or a noncoding sequence. MutChromSeq does not rely on recombination-based genetic mapping and does not exclude any DNA sequence from being targeted.
References provided by Crossref.org
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