Rapid Gene Isolation Using MutChromSeq
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
Grantová podpora
Biotechnology and Biological Sciences Research Council - United Kingdom
- Klíčová slova
- Chromosome isolation, FISHIS, Flow cytometry and chromosome sorting, Gene cloning, Mutational genomics,
- MeSH
- chromozomy rostlin genetika MeSH
- DNA rostlinná genetika MeSH
- genom rostlinný MeSH
- genomika metody MeSH
- hybridizace in situ fluorescenční metody MeSH
- mutace * MeSH
- polyploidie MeSH
- průtoková cytometrie metody MeSH
- regulace genové exprese u rostlin MeSH
- rostliny genetika MeSH
- sekvenční analýza DNA metody MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- DNA rostlinná MeSH
MutChromSeq is an approach for isolation of genes and DNA sequences controlling gene expression in plants with complex and polyploid genomes. It involves a lossless complexity reduction by flow cytometric chromosome sorting and shotgun sequencing DNA from isolated chromosomes. Comparison of sequences from wild-type parental chromosome with chromosomes from multiple independently derived mutants identifies causative mutations in a single candidate gene or a noncoding sequence. MutChromSeq does not rely on recombination-based genetic mapping and does not exclude any DNA sequence from being targeted.
Citace poskytuje Crossref.org
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