A green tea polyphenol epigallocatechin-3-gallate enhances neuroregeneration after spinal cord injury by altering levels of inflammatory cytokines
Jazyk angličtina Země Anglie, Velká Británie Médium print-electronic
Typ dokumentu časopisecké články
PubMed
28899730
DOI
10.1016/j.neuropharm.2017.09.006
PII: S0028-3908(17)30420-3
Knihovny.cz E-zdroje
- Klíčová slova
- Cytokines, EGCG, Green tea, Neuroprotection, Neuroregeneration, Spinal cord injury, p65NFkB,
- MeSH
- axony účinky léků MeSH
- čaj chemie MeSH
- chování zvířat účinky léků MeSH
- cytokiny metabolismus MeSH
- katechin aplikace a dávkování analogy a deriváty MeSH
- mediátory zánětu metabolismus MeSH
- myelitida komplikace metabolismus MeSH
- neuroprotektivní látky aplikace a dávkování MeSH
- NF-kappa B metabolismus MeSH
- poranění míchy komplikace metabolismus patologie prevence a kontrola MeSH
- potkani Wistar MeSH
- regenerace nervu účinky léků MeSH
- signální transdukce účinky léků MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- čaj MeSH
- cytokiny MeSH
- epigallocatechin gallate MeSH Prohlížeč
- katechin MeSH
- mediátory zánětu MeSH
- neuroprotektivní látky MeSH
- NF-kappa B MeSH
Spinal cord injury (SCI) is a debilitating condition which is characterized by an extended secondary injury due to the presence of inflammatory local milieu. Epigallocatechin gallate (EGCG) appears to possess strong neuroprotective properties. Here, we evaluated the beneficial effect of EGCG on recovery from SCI. Male Wistar rats were given either EGCG or saline directly to the injured spinal cord and thereafter a daily IP injection. Behavior recovery was monitored by BBB, plantar, rotarod and flat-beam tests. The levels of inflammatory cytokines were determined on days 1, 3, 7, 10 and 14 after SCI. Additionally, NF-κB pathway activity was evaluated. The results demonstrated that EGCG-treated rats displayed a superior behavioral performance in a flat beam test, higher axonal sprouting and positive remodelation of glial scar. Cytokine analysis revealed a reduction in IL-6, IL2, MIP1α and RANTES levels on days 1 and 3, and an upregulation of IL-4, IL-12p70 and TNFα 1 day following SCI in EGCG-treated rats. Treatment with EGCG was effective in decreasing the nuclear translocation of subunit p65 (RelA) of the NF-κB dimer, and therefore canonical NF-κB pathway attenuation. A significant increase in the gene expression of growth factors (FGF2 and VEGF), was noted in the spinal cord of EGCG-treated rats. Further, EGCG influenced expression of M1 and M2 macrophage markers. Our results have demonstrated a therapeutic value of EGCG in SCI, as observed by better behavioral performance measured by flat beam test, modulation of inflammatory cytokines and induction of higher axonal sprouting.
Citace poskytuje Crossref.org
Biomaterials and Magnetic Stem Cell Delivery in the Treatment of Spinal Cord Injury
