Strong antimicrobial activity of xanthohumol and other derivatives from hops (Humulus lupulus L.) on gut anaerobic bacteria
Language English Country Denmark Media print-electronic
Document type Journal Article
PubMed
28960474
DOI
10.1111/apm.12747
Knihovny.cz E-resources
- Keywords
- Clostridium, Xanthohumol, anaerobic bacteria, gut,
- MeSH
- Anaerobiosis physiology MeSH
- Anti-Bacterial Agents isolation & purification pharmacology MeSH
- Bacteroides fragilis drug effects growth & development MeSH
- Clostridioides difficile drug effects growth & development pathogenicity MeSH
- Clostridium perfringens drug effects growth & development MeSH
- Cyclohexenes isolation & purification pharmacology MeSH
- Flavonoids isolation & purification pharmacology MeSH
- Humulus chemistry MeSH
- Cross Infection microbiology MeSH
- Humans MeSH
- Microbial Sensitivity Tests MeSH
- Propiophenones isolation & purification pharmacology MeSH
- Diarrhea microbiology MeSH
- Enterocolitis, Pseudomembranous microbiology MeSH
- Plant Extracts chemistry MeSH
- Symbiosis physiology MeSH
- Terpenes isolation & purification pharmacology MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Anti-Bacterial Agents MeSH
- Cyclohexenes MeSH
- Flavonoids MeSH
- humulon MeSH Browser
- lupulon MeSH Browser
- Propiophenones MeSH
- Plant Extracts MeSH
- Terpenes MeSH
- xanthohumol MeSH Browser
Anaerobic bacteria, such as Bacteroides fragilis or Clostridium perfringens, are part of indigenous human flora. However, Clostridium difficile represents also an important causative agent of nosocomial infectious antibiotic-associated diarrhoea. Treatment of C. difficile infection is problematic, making it imperative to search for new compounds with antimicrobial properties. Hops (Humulus lupulus L.) contain substances with antibacterial properties. We tested antimicrobial activity of purified hop constituents humulone, lupulone and xanthohumol against anaerobic bacteria. The antimicrobial activity was established against B. fragilis, C. perfringens and C. difficile strains according to standard testing protocols (CLSI, EUCAST), and the minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBC) were calculated. All C. difficile strains were toxigenic and clinically relevant, as they were isolated from patients with diarrhoea. Strongest antimicrobial effects were observed with xanthohumol showing MIC and MBC values of 15-107 μg/mL, which are close to those of conventional antibiotics in the strains of bacteria with increased resistance. Slightly higher MIC and MBC values were obtained with lupulone followed by higher values of humulone. Our study, thus, shows a potential of purified hop compounds, especially xanthohumol, as alternatives for treatment of infections caused by select anaerobic bacteria, namely nosocomial diarrhoea caused by resistant strains.
Faculty of Military Medical Sciences University of Defence Hradec Kralove Czech Republic
Faculty of Pharmacy Charles University Hradec Králové Czech Republic
Institute of Microbiology Czech Academy of Sciences Prague 4 Czech Republic
Research Institute of Brewing and Malting PLC Prague Czech Republic
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