Mobile phase effects on the retention on polar columns with special attention to the dual hydrophilic interaction-reversed-phase liquid chromatography mechanism, a review
Language English Country Germany Media print-electronic
Document type Journal Article, Review
- Keywords
- dual retention mechanism, gradient elution, hydrophilic interaction liquid chromatography, two-dimensional separation, water adsorption,
- MeSH
- Chromatography, Liquid methods MeSH
- Chromatography, Reverse-Phase methods MeSH
- Diffusion MeSH
- Hydrophobic and Hydrophilic Interactions MeSH
- Indicators and Reagents MeSH
- Ions MeSH
- Linear Models MeSH
- Organic Chemicals MeSH
- Silicon Dioxide MeSH
- Polymers chemistry MeSH
- Solvents MeSH
- Static Electricity MeSH
- Water chemistry MeSH
- Publication type
- Journal Article MeSH
- Review MeSH
- Names of Substances
- Indicators and Reagents MeSH
- Ions MeSH
- Organic Chemicals MeSH
- Silicon Dioxide MeSH
- Polymers MeSH
- Solvents MeSH
- Water MeSH
Hydrophilic interaction liquid chromatography on polar columns in aqueous-organic mobile phases has become increasingly popular for the separation of many biologically important compounds in chemical, environmental, food, toxicological, and other samples. In spite of many new applications appearing in literature, the retention mechanism is still controversial. This review addresses recent progress in understanding of the retention models in hydrophilic interaction liquid chromatography. The main attention is focused on the role of water, both adsorbed by the column and contained in the bulk mobile phase. Further, the theoretical retention models in the isocratic and gradient elution modes are discussed. The dual hydrophilic interaction liquid chromatography reversed-phase retention mechanism on polar columns is treated in detail, especially with respect to the practical use in one- and two-dimensional liquid chromatography separations.
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