Historic and geographic surveillance of Pseudogymnoascus destructans possible from collections of bat parasites
Language English Country Germany Media print-electronic
Document type Journal Article
PubMed
29181887
DOI
10.1111/tbed.12773
Knihovny.cz E-resources
- Keywords
- Chiroptera, Eurasia, Russia, ectoparasite, fungal infection, white-nose syndrome,
- MeSH
- Ascomycota genetics isolation & purification pathogenicity MeSH
- Chiroptera parasitology MeSH
- Arthropod Vectors microbiology MeSH
- Diptera microbiology MeSH
- DNA, Fungal genetics MeSH
- Ectoparasitic Infestations epidemiology MeSH
- Real-Time Polymerase Chain Reaction MeSH
- Mycoses epidemiology MeSH
- Parasite Load MeSH
- Tissue Preservation MeSH
- Geography MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Geographicals
- Czech Republic epidemiology MeSH
- Russia epidemiology MeSH
- Names of Substances
- DNA, Fungal MeSH
Specimens archived in wet collections represent valuable material for scientific research. Here, we show that bat fly (Diptera, Nycteribiidae) samples contain DNA of Pseudogymnoascus destructans, a fungus pathogenic to bats. Using dual-probe quantitative PCR, we detected P. destructans DNA on bat flies collected in the Samara, Sverdlovsk and Irkutsk regions of Russia between 2005 and 2017. Fungal load was significantly lower on bat flies from wet collections than on freshly collected mites in the Czech Republic. The bat pathogen was present in the Samara region (European part of Russia) in 2005, that is, a year before recognition of white-nose syndrome in North America. As Samara and Irkutsk regions were identified as new positive locations of P. destructans, our data expand the known geographic distribution of P. destructans. We conclude that ethanol-stored ectoparasites can be used to identify the presence of pathogens in historic bat populations and understudied geographical regions.
Department of Biochemistry Ural State Medical University Ekaterinburg Russia
Department of Botany and Zoology Masaryk University Brno Czech Republic
Institute of Biostatistics and Analyses Masaryk University Brno Czech Republic
Institute of Vertebrate Biology Czech Academy of Sciences Brno Czech Republic
Laboratory of Biodiversity Monitoring National Research Tomsk State University Tomsk Russia
References provided by Crossref.org
Active surveillance for antibodies confirms circulation of lyssaviruses in Palearctic bats
White-nose syndrome detected in bats over an extensive area of Russia
Alterations in the health of hibernating bats under pathogen pressure
