A more sensitive detection of micrometastases of NSCLC in lymph nodes using the one-step nucleic acid amplification (OSNA) method
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články
PubMed
29205350
DOI
10.1002/jso.24826
Knihovny.cz E-zdroje
- Klíčová slova
- H&E, OSNA assay, cytokeratin 19, lung cancer, pTNM,
- MeSH
- adenokarcinom genetika sekundární chirurgie MeSH
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- lymfatické uzliny metabolismus patologie chirurgie MeSH
- messenger RNA MeSH
- mikrometastázy MeSH
- nádorové biomarkery genetika MeSH
- nádory plic genetika patologie chirurgie MeSH
- následné studie MeSH
- nemalobuněčný karcinom plic genetika sekundární chirurgie MeSH
- prognóza MeSH
- prospektivní studie MeSH
- senioři MeSH
- spinocelulární karcinom genetika sekundární chirurgie MeSH
- techniky amplifikace nukleových kyselin metody MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- messenger RNA MeSH
- nádorové biomarkery MeSH
BACKGROUND: Detection of tumor cells in lymph nodes (LNs) removed during the treatment of pulmonary tumor by radical surgery is limited by the possibilities of standard histopathological methods. The goal of this study was to obtain more accurate pTNM status by a more sensitive detection of micrometastases in LNs. METHODS: A total of 885 LNs, an average of 13.8 LNs per patient, were removed during 64 surgeries. LNs from the same zone were pooled together as a group, five groups of LNs were examined in each patient. A total of 320 groups of LNs were examined. One-step nucleic acid amplification (OSNA) method was compared to standard histopathological examination with haematoxylin-eosin (H&E) staining and CK19 immunohistochemistry, specifically by an ultimate analysis of all intraoperatively removed LNs. RESULTS: Identical results for H&E and OSNA examinations were recorded in 286 groups of LNs (89.4%). In total, positive examinations were recorded in 27 groups of LNs (8.4%) using the OSNA method, which were H&E negative. In seven groups of LNs (2.2%), the H&E examination was positive, while OSNA method produced negative results. CONCLUSIONS: The OSNA examination led to a higher pTNM stage classification in 14 (21.9%) patients. The clinical significance remains the subject of follow-up research.
Biomedical Centre Charles University Pilsen Czech Republic
Department of Biology Charles University Pilsen Czech Republic
Department of Medical Chemistry and Biochemistry Charles University Pilsen Czech Republic
Department of Surgery University Hospital in Pilsen Pilsen Czech Republic
Sikl's Department of Pathology University Hospital in Pilsen Pilsen Czech Republic
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