A more sensitive detection of micrometastases of NSCLC in lymph nodes using the one-step nucleic acid amplification (OSNA) method
Language English Country United States Media print-electronic
Document type Journal Article
PubMed
29205350
DOI
10.1002/jso.24826
Knihovny.cz E-resources
- Keywords
- H&E, OSNA assay, cytokeratin 19, lung cancer, pTNM,
- MeSH
- Adenocarcinoma genetics secondary surgery MeSH
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Lymph Nodes metabolism pathology surgery MeSH
- RNA, Messenger MeSH
- Neoplasm Micrometastasis MeSH
- Biomarkers, Tumor genetics MeSH
- Lung Neoplasms genetics pathology surgery MeSH
- Follow-Up Studies MeSH
- Carcinoma, Non-Small-Cell Lung genetics secondary surgery MeSH
- Prognosis MeSH
- Prospective Studies MeSH
- Aged MeSH
- Carcinoma, Squamous Cell genetics secondary surgery MeSH
- Nucleic Acid Amplification Techniques methods MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- RNA, Messenger MeSH
- Biomarkers, Tumor MeSH
BACKGROUND: Detection of tumor cells in lymph nodes (LNs) removed during the treatment of pulmonary tumor by radical surgery is limited by the possibilities of standard histopathological methods. The goal of this study was to obtain more accurate pTNM status by a more sensitive detection of micrometastases in LNs. METHODS: A total of 885 LNs, an average of 13.8 LNs per patient, were removed during 64 surgeries. LNs from the same zone were pooled together as a group, five groups of LNs were examined in each patient. A total of 320 groups of LNs were examined. One-step nucleic acid amplification (OSNA) method was compared to standard histopathological examination with haematoxylin-eosin (H&E) staining and CK19 immunohistochemistry, specifically by an ultimate analysis of all intraoperatively removed LNs. RESULTS: Identical results for H&E and OSNA examinations were recorded in 286 groups of LNs (89.4%). In total, positive examinations were recorded in 27 groups of LNs (8.4%) using the OSNA method, which were H&E negative. In seven groups of LNs (2.2%), the H&E examination was positive, while OSNA method produced negative results. CONCLUSIONS: The OSNA examination led to a higher pTNM stage classification in 14 (21.9%) patients. The clinical significance remains the subject of follow-up research.
Biomedical Centre Charles University Pilsen Czech Republic
Department of Biology Charles University Pilsen Czech Republic
Department of Medical Chemistry and Biochemistry Charles University Pilsen Czech Republic
Department of Surgery University Hospital in Pilsen Pilsen Czech Republic
Sikl's Department of Pathology University Hospital in Pilsen Pilsen Czech Republic
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