Implementing CRISPR-Cas technologies in conventional and non-conventional yeasts: Current state and future prospects
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't, Review
PubMed
29331410
DOI
10.1016/j.biotechadv.2018.01.006
PII: S0734-9750(18)30006-5
Knihovny.cz E-resources
- Keywords
- CRISPR-Cas, Candida albicans/glabrata, Expression optimization, Metabolic engineering, Pichia pastoris, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Synthetic biology, Yarrowia lipolytica, Yeasts,
- MeSH
- Point Mutation MeSH
- Chromosomes, Fungal MeSH
- CRISPR-Cas Systems * MeSH
- Gene Editing methods MeSH
- Microorganisms, Genetically-Modified MeSH
- Cloning, Molecular MeSH
- Yeasts genetics MeSH
- Metabolic Engineering MeSH
- Pichia genetics MeSH
- Gene Expression Regulation, Fungal MeSH
- Saccharomyces cerevisiae genetics MeSH
- Gene Drive Technology MeSH
- RNA, Guide, CRISPR-Cas Systems MeSH
- Yarrowia genetics MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Review MeSH
- Names of Substances
- RNA, Guide, CRISPR-Cas Systems MeSH
Within five years, the CRISPR-Cas system has emerged as the dominating tool for genome engineering, while also changing the speed and efficiency of metabolic engineering in conventional (Saccharomyces cerevisiae and Schizosaccharomyces pombe) and non-conventional (Yarrowia lipolytica, Pichia pastoris syn. Komagataella phaffii, Kluyveromyces lactis, Candida albicans and C. glabrata) yeasts. Especially in S. cerevisiae, an extensive toolbox of advanced CRISPR-related applications has been established, including crisprTFs and gene drives. The comparison of innovative CRISPR-Cas expression strategies in yeasts presented here may also serve as guideline to implement and refine CRISPR-Cas systems for highly efficient genome editing in other eukaryotic organisms.
References provided by Crossref.org
Production and secretion dynamics of prokaryotic Penicillin G acylase in Pichia pastoris
