Long-term exposure to very low doses of bisphenol S affects female reproduction
Language English Country Great Britain, England Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
29748175
DOI
10.1530/rep-18-0092
PII: REP-18-0092
Knihovny.cz E-resources
- MeSH
- Chorionic Gonadotropin pharmacology MeSH
- Endocrine Disruptors administration & dosage MeSH
- Phenols administration & dosage MeSH
- Fertilization drug effects MeSH
- Gonadotropins, Equine pharmacology MeSH
- Immunohistochemistry MeSH
- Mice, Inbred ICR MeSH
- Mice MeSH
- Oocytes drug effects metabolism MeSH
- Ovarian Follicle drug effects metabolism MeSH
- Ovary drug effects metabolism MeSH
- Proteome drug effects MeSH
- Reproduction drug effects MeSH
- Sulfones administration & dosage MeSH
- Tandem Mass Spectrometry MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- bisphenol S MeSH Browser
- Chorionic Gonadotropin MeSH
- Endocrine Disruptors MeSH
- Phenols MeSH
- Gonadotropins, Equine MeSH
- Proteome MeSH
- Sulfones MeSH
Bisphenols belong to the endocrine disruptors, affecting reproduction even in extremely low doses. Bisphenol S (BPS) has become widely used as a substitute for the earlier-used bisphenol A; however, its harmlessness is questionable. The aim of this study was to evaluate the effect of BPS on folliculogenesis and oocyte quality after in vivo exposure to low doses of BPS. Four-week-old ICR females (n = 16 in each experimental group) were exposed to vehicle control (VC), BPS1 (0.001 ng BPS.g/bw/day), BPS2 (0.1 ng.g/bw/day), BPS3 (10 ng.g/bw/day) and BPS4 (100 ng.g/bw/day) for 4 weeks. Ovaries were subjected to stereology and nano liquid chromatography-mass spectrometry (LC/MS). Simultaneously, metaphase II oocytes were obtained after pregnant mare serum gonadotrophin and human chorionic gonadotrophin administration, followed by immunostaining. In particular, mating and two-cell embryo flushing were performed. We observed that BPS decreases the amount of ovarian follicles and BPS2 (0.1 ng.g/bw/day) affects the volume of antral follicles. Accordingly, ovarian proteome is affected after BPS2 treatment. While BPS2 dosing results mainly in cytoskeletal damage in matured oocytes, the effects of BPS3 and BPS4 seem to be due instead to epigenetic alterations in oocytes. Arguably, these changes lead to observed affection of in vivo fertilization rate after BPS3 and BPS4 treatment. BPS significantly affects female reproduction astoundingly in extremely low doses. These findings underline the necessity to assess the risk of ongoing BPS exposure for public health.
Biomedical CenterFaculty of Medicine in Pilsen Charles University Pilsen Czech Republic
Institute of Animal SciencePrague Czech Republic
Université Lille1Sciences et Technologies FR3688 CNRS Villeneuve d'Ascq Cedex France
References provided by Crossref.org
Evidence of endogenously produced hydrogen sulfide (H2S) and persulfidation in male reproduction
Nursing Exposure to Bisphenols as a Cause of Male Idiopathic Infertility
Low doses of Bisphenol S affect post-translational modifications of sperm proteins in male mice
