Bisphenol S (BPS), the main replacement for bisphenol A (BPA), is thought to be toxic, but limited information is available on the effects of Bisphenol S on ovarian follicles. In our study, we demonstrated the presence of Bisphenol S in the follicular fluid of women at a concentration of 22.4 nM. The effect of such concentrations of Bisphenol S on oocyte maturation and subsequent embryo development is still unknown. Therefore, we focused on the effect of Bisphenol S on in vitro oocyte maturation, fertilization, and embryo development. As a model, we used porcine oocytes, which show many physiological similarities to human oocytes. Oocytes were exposed to Bisphenol S concentrations similar to those detected in female patients in the ART clinic. We found a decreased ability of oocytes to successfully complete meiotic maturation. Mature oocytes showed an increased frequency of meiotic spindle abnormalities and chromosome misalignment. Alarming associations of oocyte Bisphenol S exposure with the occurrence of aneuploidy and changes in the distribution of mitochondria and mitochondrial proteins were demonstrated for the first time. However, the number and quality of blastocysts derived from oocytes that successfully completed meiotic maturation under the influence of Bisphenol S was not affected.

Biomedical Center Faculty of Medicine in Pilsen Charles University Pilsen Czechia

Centre for Research on Diabetes Metabolism and Nutrition 3rd Faculty of Medicine Charles University Prague Czechia

Department of Agricultural Education College of Education Sunchon National University Suncheon Republic of Korea

Department of Biochemistry Cell and Molecular Biology 3rd Faculty of Medicine Charles University Prague Czechia

Department of Biology of Reproduction Institute of Animal Science Prague Czechia

Department of Histology and Embryology Faculty of Medicine in Pilsen Charles University Pilsen Czechia

Department of Obstetrics and Gynecology Faculty of Medicine Masaryk University and University Hospital Brno Brno Czechia

Department of Pathophysiology 3rd Faculty of Medicine Charles University Prague Czechia

Faculty of Natural Sciences and Informatics Constantine the Philosopher University of Nitra Nitra Slovakia

Institute of Biology and Medical Genetics 1st Faculty of Medicine Charles University Prague Czechia

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