Exposure to bisphenols is related to negative effects on male reproduction. The bisphenols exposure is associated with several modes of action including negative impact on the blood-testis barrier (BTB) in testes or direct effect on spermatozoa. Bisphenols have been detected in human seminal plasma, but the possible mechanism of seminal transfer of bisphenols is not clear. Some authors consider the transfer through the blood-testis barrier to be crucial. Therefore, in this work, we compared normozoospermic men and men after vasectomy who have interrupted vas deferens and their ejaculate does not contain testicular products. We measured the concentration of bisphenol A (BPA), bisphenol S (BPS) and bisphenol F (BPF) in the urine and seminal plasma of these men using liquid chromatography tandem mass spectrometry (LC/MSMS). We found that the ratio of urinary and seminal plasma content of bisphenols did not differ in normozoospermic men or men after vasectomy. From the obtained data, it can be concluded that the pathways of transport of bisphenols into seminal plasma are not primarily through the testicular tissue, but this pathway is applied similarly to other routes of transmission by a corresponding ejaculate volume ratio. To a much greater extent than through testicular tissue, bisphenols enter the seminal plasma mainly as part of the secretions of the accessory glands.

Center of Assisted Reproduction Department of Gynecology and Obstetrics Masaryk University Brno and University Hospital Brno 62500 Brno Czech Republic

Department of Anatomy Poznan University of Medical Sciences 61 701 Poznan Poland

Department of Health Sciences Faculty of Medicine Masaryk University 62500 Brno Czech Republic

Department of Histology and Embryology Poznan University of Medical Sciences 61 701 Poznan Poland

Department of Urology Faculty of Medicine Masaryk University Brno and University Hospital Brno 62500 Brno Czech Republic

Department of Veterinary Sciences Czech University of Life Sciences Prague 16500 Prague Czech Republic

Department of Veterinary Surgery Institute of Veterinary Medicine Nicolaus Copernicus University 87 100 Torun Poland

Prestage Department of Poultry Sciences North Carolina State University Raleigh NC 27695 USA

RECETOX Centre Faculty of Science Masaryk University 62500 Brno Czech Republic

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