Exposure to alternative bisphenols BPS and BPF through breast milk: Noxious heritage effect during nursing associated with idiopathic infertility
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
33476676
DOI
10.1016/j.taap.2021.115409
PII: S0041-008X(21)00016-8
Knihovny.cz E-zdroje
- Klíčová slova
- Bisphenol, Epigenetics, Oocyte maturation, Spindle formation,
- MeSH
- aparát dělícího vřeténka účinky léků metabolismus patologie MeSH
- benzhydrylové sloučeniny metabolismus toxicita MeSH
- epigeneze genetická MeSH
- fenoly metabolismus toxicita MeSH
- fertilita účinky léků MeSH
- hodnocení rizik MeSH
- IVM techniky MeSH
- kojená zvířata MeSH
- laktace metabolismus MeSH
- matka - expozice noxám MeSH
- mléko metabolismus MeSH
- myši inbrední ICR MeSH
- myši MeSH
- oocyty účinky léků metabolismus patologie MeSH
- ovariální rezerva účinky léků MeSH
- ovarium účinky léků metabolismus patofyziologie MeSH
- sulfony metabolismus toxicita MeSH
- těhotenství MeSH
- vývojová regulace genové exprese MeSH
- ženská infertilita chemicky indukované metabolismus patologie patofyziologie MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- těhotenství MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- benzhydrylové sloučeniny MeSH
- bisphenol F MeSH Prohlížeč
- bisphenol S MeSH Prohlížeč
- fenoly MeSH
- sulfony MeSH
There is increasing evidence that bisphenols BPS and BPF, which are analogues of BPA, have deleterious effects on reproduction even at extremely low doses. Indirect exposure via the maternal route (i.e. across the placenta and/or by breastfeeding) is underestimated, although it can be assumed to be a cause of idiopathic female infertility. Therefore, we hypothesised the deleterious effects of exposure to BPA analogues during breastfeeding on the ovarian and oocyte quality of offspring. A 15-day exposure period of pups was designed, whilst nursing dams (N ≥ 6 per experimental group) were treated via drinking water with a low (0.2 ng/g body weight/day) or moderate (20 ng/g body weight/day) dose of bisphenol, mimicking real exposure in humans. Thereafter, female pups were bred to 60 days and oocytes were collected. Immature oocytes were used in the in-vitro maturation assay; alternatively, in-vivo-matured oocytes were isolated and used for parthenogenetic activation. Both in-vitro- and in-vivo-matured oocytes were subjected to immunostaining of spindle microtubules (α-tubulin) and demethylation of histone H3 on the lysine K27 (H3K27me2) residue. Although very low doses of both BPS and BPF did not affect the quality of ovarian histology, spindle formation and epigenetic signs were affected. Notably, in-vitro-matured oocytes were significantly sensitive to both doses of BPS and BPF. Although no significant differences in spindle-chromatin quality were identified in ovulated and in-vivo-matured oocytes, developmental competence was significantly damaged. Taken together, our mouse model provides evidence that bisphenol analogues represent a risk to human reproduction, possibly leading to idiopathic infertility in women.
Biomedical Center Faculty of Medicine in Pilsen Charles University Pilsen Czech Republic
Institute of Animal Science Prague 10 Uhrineves Czech Republic
Citace poskytuje Crossref.org
Nursing Exposure to Bisphenols as a Cause of Male Idiopathic Infertility
