Exposure to alternative bisphenols BPS and BPF through breast milk: Noxious heritage effect during nursing associated with idiopathic infertility
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
33476676
DOI
10.1016/j.taap.2021.115409
PII: S0041-008X(21)00016-8
Knihovny.cz E-resources
- Keywords
- Bisphenol, Epigenetics, Oocyte maturation, Spindle formation,
- MeSH
- Spindle Apparatus drug effects metabolism pathology MeSH
- Benzhydryl Compounds metabolism toxicity MeSH
- Epigenesis, Genetic MeSH
- Phenols metabolism toxicity MeSH
- Fertility drug effects MeSH
- Risk Assessment MeSH
- In Vitro Oocyte Maturation Techniques MeSH
- Animals, Suckling MeSH
- Lactation metabolism MeSH
- Maternal Exposure MeSH
- Milk metabolism MeSH
- Mice, Inbred ICR MeSH
- Mice MeSH
- Oocytes drug effects metabolism pathology MeSH
- Ovarian Reserve drug effects MeSH
- Ovary drug effects metabolism physiopathology MeSH
- Sulfones metabolism toxicity MeSH
- Pregnancy MeSH
- Gene Expression Regulation, Developmental MeSH
- Infertility, Female chemically induced metabolism pathology physiopathology MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Pregnancy MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Benzhydryl Compounds MeSH
- bisphenol F MeSH Browser
- bisphenol S MeSH Browser
- Phenols MeSH
- Sulfones MeSH
There is increasing evidence that bisphenols BPS and BPF, which are analogues of BPA, have deleterious effects on reproduction even at extremely low doses. Indirect exposure via the maternal route (i.e. across the placenta and/or by breastfeeding) is underestimated, although it can be assumed to be a cause of idiopathic female infertility. Therefore, we hypothesised the deleterious effects of exposure to BPA analogues during breastfeeding on the ovarian and oocyte quality of offspring. A 15-day exposure period of pups was designed, whilst nursing dams (N ≥ 6 per experimental group) were treated via drinking water with a low (0.2 ng/g body weight/day) or moderate (20 ng/g body weight/day) dose of bisphenol, mimicking real exposure in humans. Thereafter, female pups were bred to 60 days and oocytes were collected. Immature oocytes were used in the in-vitro maturation assay; alternatively, in-vivo-matured oocytes were isolated and used for parthenogenetic activation. Both in-vitro- and in-vivo-matured oocytes were subjected to immunostaining of spindle microtubules (α-tubulin) and demethylation of histone H3 on the lysine K27 (H3K27me2) residue. Although very low doses of both BPS and BPF did not affect the quality of ovarian histology, spindle formation and epigenetic signs were affected. Notably, in-vitro-matured oocytes were significantly sensitive to both doses of BPS and BPF. Although no significant differences in spindle-chromatin quality were identified in ovulated and in-vivo-matured oocytes, developmental competence was significantly damaged. Taken together, our mouse model provides evidence that bisphenol analogues represent a risk to human reproduction, possibly leading to idiopathic infertility in women.
Biomedical Center Faculty of Medicine in Pilsen Charles University Pilsen Czech Republic
Institute of Animal Science Prague 10 Uhrineves Czech Republic
References provided by Crossref.org
Nursing Exposure to Bisphenols as a Cause of Male Idiopathic Infertility
