Novel approaches to study coronary vasculature development in mice
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
29770532
DOI
10.1002/dvdy.24637
Knihovny.cz E-resources
- Keywords
- Coronary artery anatomy, GFP, connexin40, coronary anomalies, embryonic development, heart,
- MeSH
- Embryo, Mammalian MeSH
- Microscopy, Confocal methods MeSH
- Coronary Vessels anatomy & histology growth & development MeSH
- Mice MeSH
- Heart Septum MeSH
- Imaging, Three-Dimensional methods MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
BACKGROUND: Coronary artery development is an intensely studied field. Mice are a popular genetic model for developmental studies, but there is no widely accepted protocol for high-throughput, high-resolution imaging of their developmental and adult coronary artery anatomy. RESULTS: Using tissue-clearing protocols and confocal microscopy, we have analyzed embryonic and juvenile mouse hearts in Cx40:GFP knock-in models with a special focus on septal artery development. We found that the septal artery, which supplies the interventricular septum, was initially formed as an arterial plexus that connected to both the left and right coronary arteries. During development, the plexus was remodeled into a single tube, which then remained connected only to the right coronary artery. Since optical imaging became limited at postnatal stages, it was supplemented with injection techniques using India ink and Microfil; the latter was subsequently analyzed with micro-CT to visualize the anatomy of coronary vessels in 3D. CONCLUSIONS: The techniques described here enable us to study the finer details of coronary artery development in mice and can, therefore, be implemented to study the pathogenesis of coronary malformations in various mouse models. Developmental Dynamics 247:1018-1027, 2018. © 2018 Wiley Periodicals, Inc.
Institute of Anatomy 1st Faculty of Medicine Charles University Prague Czech Republic
Institute of Dental Medicine 1st Faculty of Medicine Charles University Prague Czech Republic
Institute of Physiology Czech Academy of Sciences Prague Czech Republic
References provided by Crossref.org
Sixty Years of Heart Research in the Institute of Physiology of the Czech Academy of Sciences
Tissue clearing and imaging methods for cardiovascular development
Three-dimensional alignment of microvasculature and cardiomyocytes in the developing ventricle
HIF-1α is required for development of the sympathetic nervous system
