Novel approaches to study coronary vasculature development in mice
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
29770532
DOI
10.1002/dvdy.24637
Knihovny.cz E-zdroje
- Klíčová slova
- Coronary artery anatomy, GFP, connexin40, coronary anomalies, embryonic development, heart,
- MeSH
- embryo savčí MeSH
- konfokální mikroskopie metody MeSH
- koronární cévy anatomie a histologie růst a vývoj MeSH
- myši MeSH
- srdeční septum MeSH
- zobrazování trojrozměrné metody MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
BACKGROUND: Coronary artery development is an intensely studied field. Mice are a popular genetic model for developmental studies, but there is no widely accepted protocol for high-throughput, high-resolution imaging of their developmental and adult coronary artery anatomy. RESULTS: Using tissue-clearing protocols and confocal microscopy, we have analyzed embryonic and juvenile mouse hearts in Cx40:GFP knock-in models with a special focus on septal artery development. We found that the septal artery, which supplies the interventricular septum, was initially formed as an arterial plexus that connected to both the left and right coronary arteries. During development, the plexus was remodeled into a single tube, which then remained connected only to the right coronary artery. Since optical imaging became limited at postnatal stages, it was supplemented with injection techniques using India ink and Microfil; the latter was subsequently analyzed with micro-CT to visualize the anatomy of coronary vessels in 3D. CONCLUSIONS: The techniques described here enable us to study the finer details of coronary artery development in mice and can, therefore, be implemented to study the pathogenesis of coronary malformations in various mouse models. Developmental Dynamics 247:1018-1027, 2018. © 2018 Wiley Periodicals, Inc.
Institute of Anatomy 1st Faculty of Medicine Charles University Prague Czech Republic
Institute of Dental Medicine 1st Faculty of Medicine Charles University Prague Czech Republic
Institute of Physiology Czech Academy of Sciences Prague Czech Republic
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