Haloalkane dehalogenases degrade halogenated compounds to corresponding alcohols by a hydrolytic mechanism. These enzymes are being intensively investigated as model systems in experimental and in silico studies of enzyme mechanism and evolution, but also hold importance as useful biocatalysts for a number of biotechnological applications. Haloalkane dehalogenases originate from various organisms including bacteria (degraders, symbionts, or pathogens), eukaryotes, and archaea. Several members of this enzyme family have been found in marine organisms. The marine environment represents a good source of enzymes with novel properties, because of its diverse living conditions. A number of novel dehalogenases isolated from marine environments show interesting characteristics such as high activity, unusually broad substrate specificity, stability, or selectivity. In this chapter, the overview of haloalkane dehalogenases from marine organisms is presented and their characteristics are summarized together with an overview of the methods for their identification and biochemical characterization.

Loschmidt Laboratories Department of Experimental Biology Research Centre for Toxic Compounds in the Environment RECETOX Faculty of Science Masaryk University Brno Czech Republic; International Clinical for Research Center St Anne's University Hospital Brno Czech Republic

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Atypical homodimerization revealed by the structure of the (S)-enantioselective haloalkane dehalogenase DmmarA from Mycobacterium marinum

Structural Analysis of the Ancestral Haloalkane Dehalogenase AncLinB-DmbA

An Ultrasensitive Fluorescence Assay for the Detection of Halides and Enzymatic Dehalogenation