Self-Assembled Thermoresponsive Polymeric Nanogels for 19F MR Imaging
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Fluorine chemistry MeSH
- HeLa Cells MeSH
- Hemolysis drug effects MeSH
- Contrast Media adverse effects chemistry MeSH
- Cells, Cultured MeSH
- Polymethacrylic Acids chemistry MeSH
- Humans MeSH
- Magnetic Resonance Imaging methods MeSH
- Mice MeSH
- Nanogels MeSH
- Polyamines chemistry MeSH
- Polyethylene Glycols chemistry MeSH
- Polyethyleneimine chemistry MeSH
- Polymerization MeSH
- Temperature MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Duxon MeSH Browser
- Fluorine MeSH
- Contrast Media MeSH
- Polymethacrylic Acids MeSH
- Nanogels MeSH
- poly(2-methyl-2-oxazoline) MeSH Browser
- Polyamines MeSH
- polyethylene glycol polyethyleneimine nanogel MeSH Browser
- Polyethylene Glycols MeSH
- Polyethyleneimine MeSH
Magnetic resonance imaging using fluorinated contrast agents (19F MRI) enables to achive highcontrast in images due to the negligible fluorine background in living tissues. In this pilot study, we developed new biocompatible, temperature-responsive, and easily synthesized polymeric nanogels containing a sufficient concentration of magnetically equivalent fluorine atoms for 19F MRI purposes. The structure of the nanogels is based on amphiphilic copolymers containing two blocks, a hydrophilic poly[ N-(2-hydroxypropyl)methacrylamide] (PHPMA) or poly(2-methyl-2-oxazoline) (PMeOx) block, and a thermoresponsive poly[ N(2,2difluoroethyl)acrylamide] (PDFEA) block. The thermoresponsive properties of the PDFEA block allow us to control the process of nanogel self-assembly upon its heating in an aqueous solution. Particle size depends on the copolymer composition, and the most promising copolymers with longer thermoresponsive blocks form nanogels of suitable size for angiogenesis imaging or the labeling of cells (approximately 120 nm). The in vitro 19F MRI experiments reveal good sensitivity of the copolymer contrast agents, while the nanogels were proven to be noncytotoxic for several cell lines.
Institute for Clinical and Experimental Medicine Vídeňská 9 Prague 4 140 21 Czech Republic
Institute of Macromolecular Chemistry AS CR v v i Heyrovského sq 2 Prague 6 162 06 Czech Republic
References provided by Crossref.org
Fluorine polymer probes for magnetic resonance imaging: quo vadis?
