Nickel-titanium alloy (nitinol, NiTi) is a biomaterial with unique thermal shape memory, superelasticity and high damping properties. Therefore NiTi has been used in medical applications. In this in vitro study, the effect of NiTi alloy (with two surface modifications - helium and hydrogen) on gene expression profile of selected interleukins (IL-1β, IL-6 and IL-8) and matrix metalloproteinases (MMP-1 and MMP-2) in human physiological osteoblasts and human osteoarthritic osteoblasts was examined to respond to a question of the different behavior of bone tissue in the implantation of metallic materials in the presence of cells affected by the osteoarthritic process. The cells were cultivated in contact with NiTi and with or without LPS (bacterial lipolysaccharide). Changes in expression of target genes were calculated by 2-ΔΔCt method. An increased gene expression of IL-1β in osteoarthritic osteoblasts, with even higher expression in cells collected directly from the metal surface was observed. In case of physiological osteoblasts, the change in expression was detected after LPS treatment in cells surrounding the disc. Higher expression levels of IL-8 were observed in osteoarthritic osteoblasts after NiTi treatment in contact with alloy, and in physiological osteoblasts without relation to location in combination of NiTi and LPS. IL-6 was slightly increased in physiological osteoblastes after application of LPS. MMP-1 expression level was obviously significantly higher in osteoarthritic osteoblasts with differences regarding the metal surface and location. MMP-2 expression was decreased in both cell lines after LPS treatment. In conclusion, results of present study show that the NiTi alloy and the treatment by LPS, especially repeated doses of LPS, change the gene expression of selected ILs and MMPs in human osteoblast cell cultures. Some of the changes were depicted solely to osteoarthritic osteoblasts.

Academy of Sciences CR Institute of Physics of Materials Zizkova 22 616 62 Brno Czech Republic

Department of Pathological Physiology Faculty of Medicine Masaryk University 625 00 Brno Czech Republic

Zobrazit více v PubMed

Semin Arthritis Rheum. 1996 Feb;25(4):254-72 PubMed

Nat Rev Rheumatol. 2011 Jan;7(1):33-42 PubMed

Nat Clin Pract Rheumatol. 2008 Mar;4(3):128-35 PubMed

Mediators Inflamm. 1992;1(4):273-9 PubMed

J Bone Miner Metab. 2009;27(4):412-23 PubMed

Genet Mol Res. 2015 Nov 23;14(4):14811-22 PubMed

Crit Rev Oral Biol Med. 1993;4(2):197-250 PubMed

Calcif Tissue Int. 2000 Aug;67(2):151-5 PubMed

J Rheumatol. 1999 Jan;26(1):166-9 PubMed

Arthritis Rheum. 2003 Jan;48(1):134-45 PubMed

Artif Cells Blood Substit Immobil Biotechnol. 2011 Jun;39(3):137-42 PubMed

J Infect Dis. 1998 Jun;177(6):1582-7 PubMed

PLoS One. 2014 Nov 12;9(11):e90683 PubMed

Arthritis Res Ther. 2013;15(5):R111 PubMed

J Immunol. 1995 May 15;154(10):5418-25 PubMed

Biomaterials. 2002 Aug;23(16):3341-6 PubMed

Clin Exp Rheumatol. 2008 Mar-Apr;26(2):295-304 PubMed

Osteoarthritis Cartilage. 2002 Jun;10(6):491-500 PubMed

J Biomed Mater Res A. 2007 Aug;82(2):403-14 PubMed

Osteoarthritis Cartilage. 2015 Nov;23(11):1946-54 PubMed

Osteoarthritis Cartilage. 2010 Jul;18(7):942-7 PubMed

Bone. 2007 Apr;40(4):919-30 PubMed

Curr Pharm Des. 2011;17(22):2241-52 PubMed

Arthritis Rheum. 2003 Dec;48(12):3452-63 PubMed

Immunol Today. 1989 Sep;10(9):299-304 PubMed

Biomaterials. 2005 Oct;26(29):5801-7 PubMed

Bull NYU Hosp Jt Dis. 2007;65(3):222-8 PubMed

Curr Pharm Des. 2013;19(35):6375-81 PubMed

Ann Rheum Dis. 2000 Jun;59(6):455-61 PubMed

Trends Immunol. 2002 Jun;23(6):301-4 PubMed

Sci Rep. 2016 Jan 07;6:18971 PubMed

Arthritis Rheumatol. 2014 Jun;66(6):1525-36 PubMed

Crit Rev Oncol Hematol. 2002 Apr;42(1):35-56 PubMed

Blood. 1991 Apr 15;77(8):1627-52 PubMed

Biorheology. 2008;45(3-4):433-8 PubMed

Acta Biomater. 2005 Nov;1(6):705-16 PubMed

Nat Rev Rheumatol. 2010 Nov;6(11):625-35 PubMed

Invest Ophthalmol Vis Sci. 1991 Jan;32(1):88-95 PubMed

Arthritis Res Ther. 2009;11(3):224 PubMed

Curr Rheumatol Rep. 2013 May;15(5):323 PubMed

Osteoarthritis Cartilage. 2010 Apr;18(4):581-92 PubMed

Pharmacol Ther. 2014 Oct;144(1):1-11 PubMed

Osteoarthritis Cartilage. 1996 Sep;4(3):163-73 PubMed

Biomed Mater Eng. 1996;6(4):267-89 PubMed

J Biomed Mater Res. 1976 Sep;10(5):695-731 PubMed

Joint Bone Spine. 2014 Mar;81(2):137-41 PubMed

J Biomed Mater Res. 1997 Jun 15;35(4):451-7 PubMed

Biochem J. 1990 Feb 1;265(3):621-36 PubMed

J Immunol. 1990 Jan 15;144(2):499-505 PubMed

J Biomed Mater Res A. 2007 Jan;80(1):159-66 PubMed

Osteoarthritis Cartilage. 2016 Oct;24(10):1769-1775 PubMed

J Immunol. 1992 Jan 15;148(2):466-73 PubMed

J Leukoc Biol. 1994 Nov;56(5):559-64 PubMed

Anim Nutr. 2016 Jun;2(2):99-104 PubMed

Materials (Basel). 2017 Jan 10;10(1):null PubMed

J Bone Miner Metab. 2007;25(4):219-25 PubMed

Cytotoxic effects and comparative analysis of Ni ion uptake by osteoarthritic and physiological osteoblasts

Effect of Ni ion release on the cells in contact with NiTi alloys