Polyhydroxyalkanoates (PHA) are microbial polyesters which accumulate as intracellular granules in numerous prokaryotes and mainly serve as storage materials; beyond this primary function, PHA also enhance the robustness of bacteria against various stress factors. We have observed that the presence of PHA in bacterial cells substantially enhances their ability to maintain cell integrity when suddenly exposed to osmotic imbalances. In the case of the non-halophilic bacterium Cupriavidus necator, the presence of PHA decreased plasmolysis-induced cytoplasmic membrane damage during osmotic up-shock, which subsequently enabled the cells to withstand subsequent osmotic downshock. In contrast, sudden induction of osmotic up- and subsequent down-shock resulted in massive hypotonic lysis of non-PHA containing cells as determined by Transmission Electron Microscopy and Thermogravimetrical Analysis. Furthermore, a protective effect of PHA against hypotonic lysis was also observed in the case of the halophilic bacterium Halomonas halophila; here, challenged PHA-rich cells were capable of retaining cell integrity more effectively than their PHA-poor counterparts. Hence, it appears that the fact that PHA granules, as an added value to their primary storage function, protect halophiles from the harmful effect of osmotic down-shock might explain why PHA accumulation is such a common feature among halophilic prokaryotes. The results of this study, apart from their fundamental importance, are also of practical biotechnological significance: because PHA-rich bacterial cells are resistant to osmotic imbalances, they could be utilized in in-situ bioremediation technologies or during enrichment of mixed microbial consortia in PHA producers under conditions of fluctuating salinity.

Faculty of Chemistry Brno University of Technology Purkynova 118 612 00 Brno Czech Republic

Institute of Chemistry NAWI Graz University of Graz Heinrichstrasse 28 3 8010 Graz Austria; ARENA Arbeitsgemeinschaft für Ressourcenschonende and Nachhaltige Technologien Inffeldgasse 21b 8010 Graz Austria

The Czech Academy of Sciences Biology Centre v v i Branisovska 31 370 05 Ceske Budejovice Czech Republic; University of South Bohemia Faculty of Science Branisovska 31 370 05 Ceske Budejovice Czech Republic

The Czech Academy of Sciences Institute of Scientific Instruments v v i Kralovopolska 147 612 64 Brno Czech Republic

Citace poskytuje Crossref.org

Polyhydroxyalkanoate involvement in stress-survival of two psychrophilic bacterial strains from the High Arctic

On the bioprotective effects of 3-hydroxybutyrate: Thermodynamic study of binary 3HB-water systems

Biotechnological Conversion of Grape Pomace to Poly(3-hydroxybutyrate) by Moderately Thermophilic Bacterium Tepidimonas taiwanensis

The First Insight into Polyhydroxyalkanoates Accumulation in Multi-Extremophilic Rubrobacter xylanophilus and Rubrobacter spartanus