Ms1 RNA increases the amount of RNA polymerase in Mycobacterium smegmatis
Jazyk angličtina Země Anglie, Velká Británie Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
Grantová podpora
13-27150P
Czech Science Foundation - International
P305/12/G034
Czech Science Foundation - International
946216
Charles University - International
VI20152020044
Ministry of the Interior of the Czech Republic - International
CZ.02.1.01/0.0/0.0/16_019/0000778
Ministry of Education, Youth and Sports of the Czech Republic within project the Center for advanced applied science - International
CZ.02.1.01/0.0/0.0/16_019/0000778
Ministry of Education, Youth and Sports of the Czech Republic - International
PubMed
30427073
DOI
10.1111/mmi.14159
Knihovny.cz E-zdroje
- MeSH
- bakteriální RNA metabolismus MeSH
- delece genu MeSH
- DNA řízené RNA-polymerasy metabolismus MeSH
- malá nekódující RNA genetika metabolismus MeSH
- Mycobacterium smegmatis enzymologie genetika růst a vývoj metabolismus MeSH
- stanovení celkové genové exprese MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- bakteriální RNA MeSH
- DNA řízené RNA-polymerasy MeSH
- malá nekódující RNA MeSH
Ms1 is a sRNA recently found in mycobacteria and several other actinobacterial species. Ms1 interacts with the RNA polymerase (RNAP) core devoid of sigma factors, which differs from 6S RNA that binds to RNAP holoenzymes containing the primary sigma factor. Here we show that Ms1 is the most abundant non-rRNA transcript in stationary phase in Mycobacterium smegmatis. The accumulation of Ms1 stems from its high-level synthesis combined with decreased degradation. We identify the Ms1 promoter, PMs1 , and cis-acting elements important for its activity. Furthermore, we demonstrate that PNPase (an RNase) contributes to the differential accumulation of Ms1 during growth. Then, by comparing the transcriptomes of wt and ΔMs1 strains from stationary phase, we reveal that Ms1 affects the intracellular level of RNAP. The absence of Ms1 results in decreased levels of the mRNAs encoding β and β' subunits of RNAP, which is also reflected at the protein level. Thus, the ΔMs1 strain has a smaller pool of RNAPs available when the transcriptional demand increases. This contributes to the inability of the ΔMs1 strain to rapidly react to environmental changes during outgrowth from stationary phase.
Faculty of Science Department of Cell Biology Charles University Prague Czech Republic
Faculty of Science Department of Genetics and Microbiology Charles University Prague Czech Republic
Military Health Institute Military Medical Agency Prague Czech Republic
Citace poskytuje Crossref.org
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