Fine structure and Molecular Phylogenetic Position of Two Marine Gregarines, Selenidium pygospionis sp. n. and S. pherusae sp. n., with Notes on the Phylogeny of Archigregarinida (Apicomplexa)
Jazyk angličtina Země Německo Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
30453272
DOI
10.1016/j.protis.2018.06.004
PII: S1434-4610(18)30066-X
Knihovny.cz E-zdroje
- Klíčová slova
- 18S rDNA, 28S rDNA, Unicellular parasites, molecular phylogeny., polychaetes, ultrastructure,
- MeSH
- Apicomplexa klasifikace genetika izolace a purifikace ultrastruktura MeSH
- elektronová mikroskopie MeSH
- fylogeneze * MeSH
- lokomoce MeSH
- mikroskopie MeSH
- Polychaeta parazitologie MeSH
- protozoální DNA chemie genetika MeSH
- ribozomální DNA chemie genetika MeSH
- RNA ribozomální 18S genetika MeSH
- RNA ribozomální 28S genetika MeSH
- RNA ribozomální 5.8S genetika MeSH
- sekvenční analýza DNA MeSH
- shluková analýza MeSH
- vodní organismy klasifikace genetika izolace a purifikace ultrastruktura MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- protozoální DNA MeSH
- ribozomální DNA MeSH
- RNA ribozomální 18S MeSH
- RNA ribozomální 28S MeSH
- RNA ribozomální 5.8S MeSH
Archigregarines are a key group for understanding the early evolution of Apicomplexa. Here we report morphological, ultrastructural, and molecular phylogenetic evidence from two archigregarine species: Selenidium pygospionis sp. n. and S. pherusae sp. n. They exhibited typical features of archigregarines. Additionally, an axial row of vacuoles of a presumably nutrient distribution system was revealed in S. pygospionis. Intracellular stages of S. pygospionis found in the host intestinal epithelium may point to the initial intracellular localization in the course of parasite development. Available archigregarine SSU (18S) rDNA sequences formed four major lineages fitting the taxonomical affiliations of their hosts, but not the morphological or biological features used for the taxonomical revision by Levine (1971). Consequently, the genus Selenidioides Levine, 1971 should be abolished. The branching order of these lineages was unresolved; topology tests rejected neither para- nor monophyly of archigregarines. We provided phylogenies based on LSU (28S) rDNA and near-complete ribosomal operon (concatenated SSU, 5.8S, LSU rDNAs) sequences including S. pygospionis sequences. Although being preliminary, they nevertheless revealed the monophyly of gregarines previously challenged by many molecular phylogenetic studies. Despite their molecular-phylogenetic heterogeneity, archigregarines exhibit an extremely conservative plesiomorphic structure; their ultrastructural key features appear to be symplesiomorphies rather than synapomorphies.
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