Large-Scale Purification of Photon-Upconversion Nanoparticles by Gel Electrophoresis for Analogue and Digital Bioassays
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Biotin chemistry MeSH
- Electrophoresis, Agar Gel methods MeSH
- Europium chemistry MeSH
- Infrared Rays MeSH
- Metal Nanoparticles chemistry radiation effects MeSH
- Luminescent Agents chemistry isolation & purification radiation effects MeSH
- Silicon Dioxide chemistry MeSH
- Streptavidin chemistry MeSH
- Particle Size MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Biotin MeSH
- Europium MeSH
- Luminescent Agents MeSH
- Silicon Dioxide MeSH
- Streptavidin MeSH
The performance of photon-upconversion nanoparticles (UCNPs) as background-free luminescent labels in bioanalytical applications strongly depends on the preparation of well-defined and water-dispersible nanoconjugates. We have exploited the separation power of agarose-gel electrophoresis to purify milligram amounts of homogeneous UCNPs covered with carboxylated silica, biotin, or streptavidin with recovery rates of 30 to 50%. Clusters containing discrete numbers of UCNPs were isolated from the gel and reanalyzed by agarose-gel electrophoresis, single-nanoparticle-upconversion microscopy, and additional complementary methods. The purified nanoconjugates improved conventional (analogue) bioaffinity assays and provided highly monodisperse conjugates for assays that rely on counting individual UCNPs (digital assays).
Department of Biochemistry Biotechnology University of Turku 20014 Turku Finland
Institute of Analytical Chemistry of the Czech Academy of Sciences 602 00 Brno Czech Republic
Institute of Physics of Materials of the Czech Academy of Sciences 616 62 Brno Czech Republic
References provided by Crossref.org
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