LC-MS/MS study of in vivo fate of hyaluronan polymeric micelles carrying doxorubicin
Jazyk angličtina Země Anglie, Velká Británie Médium print-electronic
Typ dokumentu časopisecké články
PubMed
30732797
DOI
10.1016/j.carbpol.2018.12.104
PII: S0144-8617(18)31549-2
Knihovny.cz E-zdroje
- Klíčová slova
- Biodistribution, Doxorubicin, Hyaluronan, Pharmacokinetics, Polymeric micelles,
- MeSH
- chromatografie kapalinová MeSH
- doxorubicin chemie farmakokinetika MeSH
- kyselina hyaluronová chemie MeSH
- micely * MeSH
- molekulová hmotnost MeSH
- myši MeSH
- nosiče léků chemie MeSH
- tandemová hmotnostní spektrometrie MeSH
- tkáňová distribuce MeSH
- uvolňování léčiv MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- doxorubicin MeSH
- kyselina hyaluronová MeSH
- micely * MeSH
- nosiče léků MeSH
A better understanding of in vivo behavior of nanocarriers is necessary for further improvement in their development. Here we present a novel approach, where both the matrix and the drug can be analyzed by LCMS/MS after one sample handling. The developed method was applied for the comparison of pharmacokinetic profile of free and encapsulated doxorubicin (DOX) in oleyl hyaluronan (HA-C18:1) polymeric micelles. The results indicated that nanocarriers were rapidly dissociated upon in vivo administration. Despite this fact, the administration of encapsulated DOX led to its longer circulation time and enhanced tumor targeting. This effect was not observed injecting blank HA-C18:1 micelles followed by unencapsulated DOX. Biodistribution studies and molecular weight estimation of the carrier matrix indicated relatively high stability of HA-C18:1 ester bond in bloodstream and complete elimination of the derivative within 72 h. The proposed methodology provides a novel strategy to elucidate the pharmacokinetic behavior of polysaccharide-based drug delivery systems.
Contipro a s Dolní Dobrouč 401 561 02 Dolní Dobrouč Czech Republic
The Czech Academy of Sciences Institute of Biophysics Královopolská 135 612 65 Brno Czech Republic
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