Contamination of soil by heavy metals is among the important environmental problems due to their toxicity and negative impact to human health and the environment. An effective method for cleaning the soil from heavy metals is phytoremediation using the second-generation bioenergy species Miscanthus × giganteus. The purpose of this research is to study the benefits of M. × giganteus cultivation at the soils taken from the mining and former military sites contaminated by As, Pb, Zn, Co, Ni, Cr, Cu, V, Mn, Sr, and U as well as at the soil artificially contaminated by Zn and Pb, to evaluate the physiological parameters of the plant, to establish peculiarities of the phytoremediation process, and to characterize the behavior of the plant in relation to the nature and concentrations of the metals in the soils. Results showed that M. × giganteus was resistant to heavy metals (tolerance index ≥ 1) and that the greatest portion of metals accumulated in the root system. The morphological parameters of the plant while grown on different soils are influenced by soil type and the content of contaminants. The stress effect while growing M. × giganteus on soil artificially contaminated by Zn and Pb was evaluated by measuring the content of pigments (chlorophylls a, b, and carotenoids) in the plant's leaves. The decrease in the total content of chlorophylls, Сa + b/Сcar and transpiration rate of water along with the increase in the water absorbing capacity were observed. The accumulation of heavy metals in different parts of the plant was determined; bioaccumulation coefficient and values of translocation factor were calculated. The obtained results showed that M. × giganteus was an excluder plant for nine highly toxic elements (As, Pb, Zn, Co, Ni, Cr, Cu, V, U) and an accumulator species for the moderately dangerous elements (Mn, Sr). Further research will be focused on the extraction of stable stimulated plant-growth-promoting rhizobacteria from the rhizosphere of M. × giganteus and formulation on that base the plant-bacterial associations as well as on the comparison of the plant physiological parameters, biochemical soil activity, and accumulation of heavy metals in the Miscanthus tissues between first and second vegetations.

Al Farabi Kazakh National University 71 Al Farabi Avenue Almaty Kazakhstan 050040

Center of Physical Chemical Methods of Research and Analysis Al Farabi Kazakh National University 96a Tole bi street Almaty Kazakhstan 050012

Department of Chemical Engineering Кansas State University 1005 Durland Hall Manhattan KS USA

Department of Plant Protection National University of Life and the Environmental Sciences Gerojiv Oboronu Kyiv 13 Ukraine

Department of Technical Sciences Jan Evangelista Purkyně University in Ústí nad Labem Králova Výšina 3132 7 Ústí nad Labem Czech Republic

Institute of Plant Biology and Biotechnology 45 Timiryazev st Almaty Kazakhstan 050040

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Front Plant Sci. 2017 Jun 08;8:990 PubMed

Sci Total Environ. 2006 Sep 15;368(2-3):456-64 PubMed

J Hazard Mater. 2009 Dec 15;172(1):479-84 PubMed

Adv Biochem Eng Biotechnol. 2003;78:1-50 PubMed

Ecotoxicol Environ Saf. 2016 Apr;126:111-121 PubMed

Environ Sci Pollut Res Int. 2018 Apr;25(12):12096-12106 PubMed

Environ Pollut. 2007 May;147(1):231-7 PubMed

Environ Sci Pollut Res Int. 2019 Jan;26(3):2974-2986 PubMed

J Hazard Mater. 2010 Nov 15;183(1-3):609-15 PubMed

Environ Int. 2005 Jul;31(5):755-62 PubMed

Environ Sci Pollut Res Int. 2013 Apr;20(4):2054-63 PubMed

Int J Phytoremediation. 2015;17(1-6):448-55 PubMed

J Exp Bot. 2001 Nov;52(364):2115-26 PubMed

J Environ Manage. 2014 Oct 1;143:123-34 PubMed

Environ Sci Pollut Res Int. 2017 Feb;24(5):4990-5000 PubMed

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