IncC blaKPC-2-positive plasmid characterised from ST648 Escherichia coli
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
31077860
DOI
10.1016/j.jgar.2019.05.001
PII: S2213-7165(19)30108-0
Knihovny.cz E-resources
- Keywords
- Enterobacterales, IncC plasmid, KPC, bla(VEB-1),
- MeSH
- Anti-Bacterial Agents pharmacology MeSH
- Bacterial Proteins genetics MeSH
- beta-Lactamases genetics MeSH
- Escherichia coli drug effects genetics isolation & purification MeSH
- Klebsiella Infections MeSH
- Klebsiella pneumoniae genetics MeSH
- Humans MeSH
- Microbial Sensitivity Tests MeSH
- Drug Resistance, Multiple, Bacterial genetics MeSH
- Multilocus Sequence Typing MeSH
- Plasmids * MeSH
- Gene Transfer, Horizontal MeSH
- Base Sequence MeSH
- Whole Genome Sequencing MeSH
- DNA Transposable Elements MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Greece epidemiology MeSH
- Names of Substances
- Anti-Bacterial Agents MeSH
- Bacterial Proteins MeSH
- beta-lactamase KPC-2 MeSH Browser
- beta-lactamase VEB-1, Pseudomonas aeruginosa MeSH Browser
- beta-Lactamases MeSH
- DNA Transposable Elements MeSH
OBJECTIVES: This study describes the characterisation of type 2 IncC plasmids pC-Ec20-KPC and pC-Ec2-KPC, carrying theblaKPC-2 gene, from two multiresistant Escherichia coli recovered in University Hospital of Larissa (Greece) in 2018. METHODS: E. coli strains Ec-2Lar and Ec-20Lar were recovered from rectal swabs of two patients during monthly surveillance cultures. Transfer experiments by conjugation were carried out using rifampicin-resistant E. coli A15 laboratory strain as recipient. blaKPC-carrying plasmids were characterised by S1 profiling. Isolates were typed by MLST. Whole-genome sequencing was performed using the Sequel platform. RESULTS: Both E. coli isolates, belonging to ST648, transferred blaKPC-2 to E. coli A15 by conjugation. Plasmid analysis revealed that the transconjugants harboured blaKPC-positive plasmids of different sizes. Analysis of plasmid sequences showed that in both isolates the blaKPC-2 gene was carried on a type 2 IncC plasmid (pC-Ec20-KPC and pC-Ec2-KPC, respectively). Both plasmids carried the ARI-B resistance island consisting of several resistance genes, intact and truncated copies of several mobile elements, and a 25 571-bp segment harbouring coding sequences for an iron transporter. The blaKPC-2 gene was part of transposon Tn4401a, which was bounded by 5-bp direct repeats (TCCTT) suggesting its transposition into the IncC plasmids. CONCLUSION: To our knowledge, this is the first report on complete nucleotide sequences of type 2 IncC plasmids. These findings, which hypothesise the acquisition of KPC-2-encoding transposon Tn4401a by an IncC replicon, indicate the ongoing need for molecular surveillance studies of multidrug-resistant pathogens. In addition, they underline the increasing clinical importance of the IncC plasmid family.
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