Antimicrobial peptides prevent bacterial biofilm formation on the surface of polymethylmethacrylate bone cement
Jazyk angličtina Země Anglie, Velká Británie Médium print-electronic
Typ dokumentu časopisecké články
PubMed
31107198
DOI
10.1099/jmm.0.001000
Knihovny.cz E-zdroje
- Klíčová slova
- antibiotics, antimicrobial peptides, biofilm, implant-related infections, polymethylmethacrylate bone cement,
- MeSH
- antibakteriální látky farmakologie MeSH
- antiinfekční látky farmakologie MeSH
- bakteriální adheze účinky léků MeSH
- biofilmy účinky léků růst a vývoj MeSH
- kostní cementy MeSH
- methicilin rezistentní Staphylococcus aureus účinky léků růst a vývoj MeSH
- mikrobiální testy citlivosti MeSH
- peptidy chemická syntéza farmakologie MeSH
- polymethylmethakrylát MeSH
- protézy a implantáty mikrobiologie MeSH
- Pseudomonas aeruginosa účinky léků růst a vývoj MeSH
- Staphylococcus epidermidis účinky léků růst a vývoj MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- antibakteriální látky MeSH
- antiinfekční látky MeSH
- kostní cementy MeSH
- peptidy MeSH
- polymethylmethakrylát MeSH
PURPOSE: Antibiotic-loaded polymethylmethacrylate-based bone cement has been implemented in orthopaedics to cope with implant-related infections associated with the formation of bacterial biofilms. In the context of emerging bacterial resistance to current antibiotics, we examined the efficacy of short antimicrobial peptide-loaded bone cement in inhibiting bacterial adhesion and consequent biofilm formation on its surface. METHODOLOGY: The ability of α-helical antimicrobial peptides composed of 12 amino acid residues to prevent bacterial biofilm [methicillin-resistant Staphylococcus aureus (MRSA), Staphylococcus epidermidis, Pseudomonas aeruginosa and Escherichia coli] formation on the surface of model implants made from polymethylmethacrylate-based bone cement was evaluated by colony-forming unit (c.f.u.) counting of bacteria released by sonication from the biofilms formed on their surfaces. The biofilms on model implant surfaces were also visualized by light microscopy after staining with tetrazolium dye (MTT) and by scanning electron microscopy. RESULTS: When incorporated in the implants, these peptides caused a mean reduction in the number of bacterial cells attached to implants' surfaces (by five orders of magnitude), and 88 % of these implants showed no bacterial adhesion after being exposed to growth media containing various bacteria. CONCLUSION: The results showed that the antibiofilm activity of these peptides was comparable to that of the antibiotics, but the peptides exhibited broader specificity than the antibiotics. Given the rapid development of antibiotic resistance, antimicrobial peptides show promise as a substitute for antibiotics for loading into bone cements.
Citace poskytuje Crossref.org
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