In vitro response of human ovarian cancer cells to dietary bioflavonoid isoquercitrin
Language English Country Great Britain, England Media print-electronic
Document type Journal Article
- Keywords
- Isoquercitrin, ROS, TGF-β1, apoptosis, cell viability, human, ovarian cancer,
- MeSH
- Antioxidants pharmacology MeSH
- Apoptosis drug effects MeSH
- Humans MeSH
- Cell Line, Tumor MeSH
- Ovarian Neoplasms drug therapy genetics metabolism physiopathology MeSH
- Oxidative Stress drug effects MeSH
- Quercetin analogs & derivatives pharmacology MeSH
- Reactive Oxygen Species metabolism MeSH
- Signal Transduction drug effects MeSH
- Transforming Growth Factor beta1 genetics metabolism MeSH
- Cell Survival drug effects MeSH
- Check Tag
- Humans MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Antioxidants MeSH
- isoquercitrin MeSH Browser
- Quercetin MeSH
- Reactive Oxygen Species MeSH
- TGFB1 protein, human MeSH Browser
- Transforming Growth Factor beta1 MeSH
Isoquercitrin is a dietary bioflavonoid used as a food supplement. We studied the mechanism underlying its effect in human ovarian cancer cells using OVCAR-3 cell line. Viability, survival, apoptosis, release of human transforming growth factor-β1 (TGF-β1) and TGF-β1 receptor, and intracellular reactive oxygen species (ROS) generation by OVCAR-3 cells were examined after isoquercitrin treatment at concentrations 5, 10, 25, 50, and 100 μg mL-1. AlamarBlue assay revealed that isoquercitrin did not cause any significant change (P > 0.05) in cell viability as compared to control. Apoptotic assay using flow cytometry did not find any significant change (P > 0.05) in the proportion of live, dead and apoptotic cells as compared to control. ELISA also showed that the release of human TGF-β1 and TGF-β1 receptor were not significantly (P > 0.05) affected by isoquercitrin as compared to control. Chemiluminescence assay demonstrated that lower concentrations (5, 10, and 25 μg mL-1) were able to exhibit beneficial effects by inhibiting the generation of intracellular ROS. In contrast, elevated concentrations of 50 and 100 μg mL-1 led to oxidative stress (P < 0.05). We concluded that the beneficial effect of isoquercitrin on ovarian cancer cells may be mediated by an antioxidative pathway that involves inhibition of intracellular ROS generation, thereby limiting oxidative stress.
Department of Life Science and Bioinformatics Assam University Silchar India
Institute of Microbiology Czech Academy of Sciences Prague 4 Czech Republic
Research Centre AgroBioTech Slovak University of Agriculture in Nitra Nitra Slovak Republic
St Elizabeth Cancer Institute Hospital Bratislava Slovak Republic
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