Antibacterial and antifungal activity of defensins from the Australian paralysis tick, Ixodes holocyclus
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
31445875
DOI
10.1016/j.ttbdis.2019.101269
PII: S1877-959X(19)30218-3
Knihovny.cz E-resources
- Keywords
- Antibacterial activity, Antifungal activity, Defensins, Ixodes holocyclus, Ticks,
- MeSH
- Anti-Bacterial Agents chemistry pharmacology MeSH
- Antifungal Agents chemistry pharmacology MeSH
- Candida albicans drug effects MeSH
- Defensins chemistry genetics immunology MeSH
- Fusarium drug effects MeSH
- Phylogeny MeSH
- Gram-Negative Bacteria drug effects MeSH
- Gram-Positive Bacteria drug effects MeSH
- Ixodes genetics immunology MeSH
- Arthropod Proteins chemistry genetics immunology MeSH
- Amino Acid Sequence MeSH
- Sequence Alignment MeSH
- Transcriptome MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Australia MeSH
- Names of Substances
- Anti-Bacterial Agents MeSH
- Antifungal Agents MeSH
- Defensins MeSH
- Arthropod Proteins MeSH
Tick innate immunity involves humoral and cellular responses. Among the humoral effector molecules in ticks are the defensins which are a family of small peptides with a conserved γ-core motif that is crucial for their antimicrobial activity. Defensin families have been identified in several hard and soft tick species. However, little is known about the presence and antimicrobial activity of defensins from the Australian paralysis tick Ixodes holocyclus. In this study the I. holocyclus transcriptome was searched for the presence of defensins. Unique and non-redundant defensin sequences were identified and designated as holosins 1 - 5. The antimicrobial activity of holosins 2 and 3 and of the predicted γ-cores of holosins 1-4 (HoloTickCores 1-4), was assessed using Gram-negative and Gram-positive bacteria as well as the fungus Fusarium graminearum and the yeast Candida albicans. All holosins had molecular features that are conserved in other tick defensins. Furthermore holosins 2 and 3 were very active against the Gram-positive bacteria Staphylococcus aureus and Listeria grayi. Holosins 2 and 3 were also active against F. graminearum and C. albicans and 5 μM of peptide abrogate the growth of these microorganisms. The activity of the synthetic γ-cores was lower than that of the mature defensins apart from HoloTickCore 2 which had activity comparable to mature holosin 2 against the Gram-negative bacterium Escherichia coli. This study reveals the presence of a multigene defensin family in I. holocyclus with wide antimicrobial activity.
Centre for Comparative Genomics Murdoch University Perth WA 6150 Australia
INRA UR1264 MycSA 71 Avenue Edouard Bourlaux 33882 Villenave d'Ornon cedex France
LOEWE Centre for Translational Biodiversity Genomics Senckenberganlage 25 60325 Frankfurt Germany
SaBio Instituto de Investigación de Recursos Cinegéticos IREC CSIC UCLM JCCM Ciudad Real Spain
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