Cardiovascular progenitor cells and tissue plasticity are reduced in a myocardium affected by Becker muscular dystrophy
Language English Country Great Britain, England Media electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
32138751
PubMed Central
PMC7057505
DOI
10.1186/s13023-019-1257-4
PII: 10.1186/s13023-019-1257-4
Knihovny.cz E-resources
- Keywords
- Becker muscular dystrophy, C-kit, Cardiomyopathy, Cardiovascular progenitor cells, Dystrophin, Heart failure,
- MeSH
- Cardiomyopathy, Dilated * MeSH
- Muscular Dystrophy, Duchenne * MeSH
- Dystrophin MeSH
- Stem Cells MeSH
- Humans MeSH
- Myocardium MeSH
- Check Tag
- Humans MeSH
- Male MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Dystrophin MeSH
UNLABELLED: We describe the association of Becker muscular dystrophy (BMD) derived heart failure with the impairment of tissue homeostasis and remodeling capabilities of the affected heart tissue. We report that BMD heart failure is associated with a significantly decreased number of cardiovascular progenitor cells, reduced cardiac fibroblast migration, and ex vivo survival. BACKGROUND: Becker muscular dystrophy belongs to a class of genetically inherited dystrophin deficiencies. It affects male patients and results in progressive skeletal muscle degeneration and dilated cardiomyopathy leading to heart failure. It is a relatively mild form of dystrophin deficiency, which allows patients to be on a heart transplant list. In this unique situation, the explanted heart is a rare opportunity to study the degenerative process of dystrophin-deficient cardiac tissue. Heart tissue was excised, dissociated, and analyzed. The fractional content of c-kit+/CD45- cardiovascular progenitor cells (CVPCs) and cardiac fibroblast migration were compared to control samples of atrial tissue. Control tissue was obtained from the hearts of healthy organ donor's during heart transplantation procedures. RESULTS: We report significantly decreased CVPCs (c-kit+/CD45-) throughout the heart tissue of a BMD patient, and reduced numbers of phase-bright cells presenting c-kit positivity in the dystrophin-deficient cultured explants. In addition, ex vivo CVPCs survival and cardiac fibroblasts migration were significantly reduced, suggesting reduced homeostatic support and irreversible tissue remodeling. CONCLUSIONS: Our findings associate genetically derived heart failure in a dystrophin-deficient patient with decreased c-kit+/CD45- CVPCs and their resilience, possibly hinting at a lack of cardioprotective capability and/or reduced homeostatic support. This also correlates with reduced plasticity of the explanted cardiac tissue, related to the process of irreversible remodeling in the BMD patient's heart.
Center for Cardiovascular Surgery and Transplantation Pekarska 53 Brno 65691 Czech Republic
Central European Institute of Technology Nanobiotechnology Kamenice 5 Brno 62500 Czech Republic
Department of Biology Faculty of Medicine Masaryk University Kamenice 5 Brno 62500 Czech Republic
Department of Cardiology University Hospital Brno Jihlavska 20 Brno 62500 Czech Republic
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