Effect of Graviola (Annona Muricata l.) and Ginger (Zingiber Officinale Roscoe) on Diabetes Mellitus Induced in Male Wistar Albino Rats
Language English Country Czech Republic Media print
Document type Journal Article
PubMed
32362311
DOI
10.14712/fb2019065050275
PII: file/5914/fb2019a0029.pdf
Knihovny.cz E-resources
- MeSH
- Annona chemistry MeSH
- Antioxidants metabolism MeSH
- Apoptosis drug effects MeSH
- Biomarkers blood MeSH
- Diabetes Mellitus, Experimental blood drug therapy enzymology MeSH
- Glycated Hemoglobin metabolism MeSH
- Insulin blood MeSH
- Insulin Resistance MeSH
- Liver Function Tests MeSH
- Liver drug effects physiopathology MeSH
- Blood Glucose metabolism MeSH
- Malondialdehyde metabolism MeSH
- Tumor Suppressor Protein p53 metabolism MeSH
- NF-kappa B metabolism MeSH
- Oxidative Stress drug effects MeSH
- Rats, Wistar MeSH
- Glucose Transporter Type 2 genetics metabolism MeSH
- bcl-2-Associated X Protein metabolism MeSH
- Receptor, Insulin genetics metabolism MeSH
- Gene Expression Regulation MeSH
- Plant Extracts pharmacology therapeutic use MeSH
- Tumor Necrosis Factor-alpha metabolism MeSH
- Zingiber officinale chemistry MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Antioxidants MeSH
- Biomarkers MeSH
- Glycated Hemoglobin A MeSH
- Insulin MeSH
- Blood Glucose MeSH
- Malondialdehyde MeSH
- Tumor Suppressor Protein p53 MeSH
- NF-kappa B MeSH
- Glucose Transporter Type 2 MeSH
- bcl-2-Associated X Protein MeSH
- Receptor, Insulin MeSH
- Plant Extracts MeSH
- Tumor Necrosis Factor-alpha MeSH
Annona and ginger have prominent uses in traditional medicine; their therapeutic properties have not been sufficiently explored. The ameliorative effect of Annona or ginger extracts on hyperglycaemia associated with oxidative stress, inflammation, and apoptosis in experimentally induced diabetes was addressed. Type 1 diabetes in male rats was induced by a single injection of streptozotocin (STZ; 40 mg/kg, i.p.), then Annona (100 mg/kg) or ginger (200 mg/kg) extracts were orally administered daily for 30 days. The Annona and ginger extracts ameliorated hyperglycaemia, insulin level, glycosylated haemoglobin (HbA1c) and insulin resistance (HOMA-IR) levels in the diabetic rats. The treatments significantly ameliorated liver function enzymes and total proteins; this was confirmed by histopathological examination of liver sections. Annona and ginger extracts significantly reduced elevated malondialdehyde (MDA) and restored activity of antioxidant enzymes in the liver such as glutathione peroxidase (GPx), glutathione reductase (GR), superoxide dismutase (SOD), and catalase (CAT) and the hepatic content of reduced glutathione (GSH). The oxidative stressdependent inflammation was regulated by both Annona and ginger extracts, which was indicated by down-regulation of TNF-α, NF-κB, pro-apoptotic proteins Bax, p53, and anti-apoptotic protein Bcl-2. Moreover, the expression of insulin receptor (INSR) and glucose transporter 2 (GLUT2) genes was markedly regulated by both these extracts. The results suggest that Annona and ginger extracts ameliorate the hepatic damage resulting from diabetes by advocating antioxidants and modulating apoptotic mediator proteins in the liver of diabetic rats. In conclusion, Annona and ginger extracts have a potential therapeutic effect in the treatment of diabetes and its complications.
Anatomy and Histology Department Faculty of Medicine Sana'a University Sana'a Republic of Yemen
Biology Department College of Science King Khalid University Abha Saudi Arabia
Zoology Department Faculty of Science Mansoura University Mansoura Egypt
References provided by Crossref.org
