The impact of cell-free supernatants of Lactococcus lactis subsp. lactis strains on the tyramine formation of Lactobacillus and Lactiplantibacillus strains isolated from cheese and beer
Language English Country England, Great Britain Media print-electronic
Document type Journal Article
PubMed
34119100
DOI
10.1016/j.fm.2021.103813
PII: S0740-0020(21)00078-2
Knihovny.cz E-resources
- Keywords
- Cell-free supernatants, Fermented food, Lactic acid bacteria, Lactiplantibacillus plantarum, Lactobacillus strains, Nisin, Tyramine,
- MeSH
- Anti-Bacterial Agents analysis metabolism pharmacology MeSH
- Culture Media chemistry metabolism pharmacology MeSH
- Lactobacillaceae drug effects growth & development isolation & purification MeSH
- Lactobacillus drug effects growth & development isolation & purification MeSH
- Lactococcus lactis chemistry metabolism MeSH
- Beer microbiology MeSH
- Cheese microbiology MeSH
- Tyramine analysis metabolism pharmacology MeSH
- Chromatography, High Pressure Liquid MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Anti-Bacterial Agents MeSH
- Culture Media MeSH
- Tyramine MeSH
Tyramine is one of the most toxic biogenic amines and it is produced commonly by lactic acid bacteria in fermented food products. In present study, we investigated the influence of selected nisin-producing Lactococcus lactis subsp. lactis strains and their cell-free supernatants (CFSs) on tyramine production by four Lactobacillus and two Lactiplantibacillus strains isolated from cheese and beer. Firstly, we examined the antimicrobial effect of the CFSs from twelve Lactococcus strains against tested tyramine producers by agar-well diffusion assay. Six Lactococcus strains whose CFSs showed the highest antimicrobial effect on tyramine producers were further studied. Secondly, we investigated the influence of the selected six Lactococcus strains and their respective CFSs on tyramine production by tested Lactobacillus and Lactiplantibacillus strains in MRS broth supplemented with 2 g.L-1 of l-tyrosine. Tyramine production was monitored by HPLC-UV. The tyramine formation of all tested Lactobacillus and Lactiplantibacillus strains was not detected in the presence of Lc. lactis subsp. lactis CCDM 71 and CCDM 702, and their CFSs. Moreover, the remainder of the investigated Lactococcus strains (CCDM 670, CCDM 686, CCDM 689 and CCDM 731) and their CFSs decreased tyramine production significantly (P < 0.05) - even suppressing it completely in some cases - in four of the six tested tyramine producing strains.
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